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Título: | Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT |
Autor(es): | Mollaei, Monir Suarez-Diez, Maria Sedano-Nunez, Vicente T. Boeren, Sjef Stams, Alfons Johannes Maria Plugge, Caroline M. |
Palavras-chave: | Syntrophobacter fumaroxidans Geobacter sulfurreducens coculture interspecies electron transfer propionate proteomics |
Data: | 30-Nov-2021 |
Editora: | Frontiers Media S.A. |
Revista: | Frontiers in Microbiology |
Citação: | Mollaei, Monir; Suarez-Diez, Maria; Sedano-Nunez, Vicente T.; Boeren, Sjef; Stams, A. J. M.; Plugge, Caroline M., Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT. Frontiers in Microbiology, 12(708911), 2021 |
Resumo(s): | We established a syntrophic coculture of Syntrophobacter fumaroxidans MPOB<sup>T</sup> (SF) and Geobacter sulfurreducens PCA<sup>T</sup> (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion via the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) via direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF coculture with Methanospirillum hungatei (SF-MH), which relies on HFIT, (ii) GS pure culture growing on acetate, formate, hydrogen as propionate products, and Fe(III). We noted some evidence for DIET in the SF-GS coculture, i.e., GS in the coculture showed significantly lower abundance of uptake hydrogenase (43-fold) and formate dehydrogenase (45-fold) and significantly higher abundance of proteins related to acetate metabolism (i.e., GltA; 62-fold) compared to GS pure culture. Moreover, SF in the SF-GS coculture showed significantly lower abundance of IET-related formate dehydrogenases, Fdh3 (51-fold) and Fdh5 (29-fold), and the rate of propionate conversion in SF-GS was 8-fold lower than in the SF-MH coculture. In contrast, compared to GS pure culture, we found lower abundance of pilus-associated cytochrome OmcS (2-fold) and piliA (5-fold) in the SF-GS coculture that is suggested to be necessary for DIET. Furthermore, neither visible aggregates formed in the SF-GS coculture, nor the pili-E of SF (suggested as e-pili) were detected. These findings suggest that the IET mechanism is complex in the SF-GS coculture and can be mediated by several mechanisms rather than one discrete pathway. Our study can be further useful in understanding syntrophic propionate degradation in bioelectrochemical and anaerobic digestion systems. |
Tipo: | Artigo |
Descrição: | The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/full#supplementary-material |
URI: | https://hdl.handle.net/1822/75259 |
DOI: | 10.3389/fmicb.2021.708911 |
ISSN: | 1664302X |
Versão da editora: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/full |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55112_1.pdf | 4,1 MB | Adobe PDF | Ver/Abrir |