Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/74818
Título: | Polyamide microsized particulate polyplex carriers for the 2-OMethylRNA EFG1 antisense oligonucleotide |
Autor(es): | Araújo, Daniela Braz, Joana Dencheva, Nadya Vasileva Carvalho, Isabel Henriques, Mariana Denchev, Z. Malfois, Marc Silva, Sónia Carina |
Palavras-chave: | Degradation Amides Organic polymers X-ray scattering Molecules Candida albicans filamentation antisense oligonucleotides polyplex RNA carriers polyamide 4 polyamide 6 polymer microparticles |
Data: | 30-Abr-2021 |
Editora: | American Chemical Society |
Revista: | ACS Applied Bio Materials |
Citação: | Araújo, Daniela; Braz, Joana; Dencheva, Nadya V.; Carvalho, Isabel; Henriques, Mariana; Denchev, Zlatan Z.; Malfois, Marc; Silva, Sónia Carina, Polyamide microsized particulate polyplex carriers for the 2-OMethylRNA EFG1 antisense oligonucleotide. ACS Applied Bio Materials, 4(5), 4607-4617, 2021 |
Resumo(s): | Anti-EFG1 2-OMethylRNA is an antisense oligonucleotide (ASO) that has the ability to recognize and block the EFG1 gene and to control Candida albicans filamentation. However, it is important to protect the anti-EFG1 2-OMethylRNA ASO from the environmental human body conditions and to ensure that they will be delivered to their site of action, and polyplex microparticles (MPs) represent a class of vehicles to ASO cargo with these functionalities. Thus, the goal of this work was to develop polyplexes based on porous poly(-butyrolactam) (PA4) or poly(-caprolactam) (PA6) MPs for the anti-EFG1 2-OMethylRNA ASO cargo and delivery. Two types of polyplexes were prepared with payloads of anti-EFG1 2-OMethylRNA molecules, either entrapped or immobilized on prefabricated polyamide MPs. Our data confirm that PA4 and PA6 polyplex MPs can be feasible carriers for anti-EFG1 2-OMethylRNA ASO molecules, using either the entrapment or immobilization strategies, whereby the released ASO maintains its activity against C. albicans cells. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/74818 |
DOI: | 10.1021/acsabm.1c00334 |
ISSN: | 2576-6422 |
Versão da editora: | https://pubs.acs.org/doi/10.1021/acsabm.1c00334 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54400_1.pdf Acesso restrito! | 7,97 MB | Adobe PDF | Ver/Abrir |