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https://hdl.handle.net/1822/74497Registo completo
| Campo DC | Valor | Idioma |
|---|---|---|
| dc.contributor.author | Allkja, Jontana | por |
| dc.contributor.author | Azevedo, Andreia S. | por |
| dc.date.accessioned | 2021-10-25T10:14:54Z | - |
| dc.date.issued | 2021-02-13 | - |
| dc.identifier.citation | Allkja, Jontana; Azevedo, Andreia S., Characterization of social interactions and spatial arrangement of individual bacteria in multistrain or multispecies biofilm systems using nucleic acid mimics-fluorescence in situ hybridization. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 97-109 | por |
| dc.identifier.isbn | 978-1-0716-1115-9 | por |
| dc.identifier.issn | 1064-3745 | por |
| dc.identifier.uri | https://hdl.handle.net/1822/74497 | - |
| dc.description.abstract | Biofilms are often composed of different bacterial and fungal species/strains, which form complex structures based on social interactions with each other. Fluorescence in situ hybridization (FISH) can help us identify the different species/strains present within a biofilm, and when coupled with confocal scanning laser microscopy (CSLM), it enables the visualization of the three-dimensional (3D) structure of the biofilm and the spatial arrangement of each individual species/strain within it. In this chapter, we describe the protocol for characterizing multistrain or multispecies biofilm formation using NAM-FISH and CSLM. | por |
| dc.description.sponsorship | This work was financially supported by (a) Base Funding—UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy—LEPABE—funded by national funds through the FCT/MCTES (PIDDAC); (b) Projects POCI 01-0145-FEDER-016678 (Coded-FISH) and POCI-01-0145- FEDER-03043 (CLASInVivo) funded by FEDER funds through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PIDDAC) through FCT/MCTES; (c) BioTecNorte operation (NORTE-01- 0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte; and (d) European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska—Curie grant agreement No 722467 (Print-Aid). | por |
| dc.language.iso | eng | por |
| dc.publisher | Springer | por |
| dc.relation | UIDB/00511/2020 | por |
| dc.relation | info:eu-repo/grantAgreement/EC/H2020/722467/EU | por |
| dc.rights | restrictedAccess | por |
| dc.subject | FISH | por |
| dc.subject | Nucleic acid mimics | por |
| dc.subject | PNA | por |
| dc.subject | LNA | por |
| dc.subject | 2OMe | por |
| dc.subject | Bacteria | por |
| dc.subject | Biofilm | por |
| dc.subject | Multispecies | por |
| dc.subject | Multistrain | por |
| dc.subject | Microscopy | por |
| dc.subject | CLSM | por |
| dc.title | Characterization of social interactions and spatial arrangement of individual bacteria in multistrain or multispecies biofilm systems using nucleic acid mimics-fluorescence in situ hybridization | por |
| dc.type | bookPart | por |
| dc.peerreviewed | yes | - |
| dc.relation.publisherversion | https://link.springer.com/book/10.1007/978-1-0716-1115-9 | por |
| dc.comments | CEB54198 | por |
| oaire.citationStartPage | 97 | por |
| oaire.citationEndPage | 109 | por |
| oaire.citationConferencePlace | New York, NY | - |
| oaire.citationVolume | Methods in Molecular Biology 2246 | - |
| dc.date.updated | 2021-10-25T08:44:45Z | - |
| dc.identifier.doi | 10.1007/978-1-0716-1115-9_7 | por |
| dc.date.embargo | 10000-01-01 | - |
| dc.identifier.pmid | 33576985 | por |
| dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
| sdum.journal | Methods in Molecular Biology | por |
| sdum.bookTitle | Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts | por |
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