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https://hdl.handle.net/1822/74215
Título: | Viable but non-cultivable state: a strategy for Staphylococcus aureus survivable in dual-species biofilms with Pseudomonas aeruginosa? |
Autor(es): | Magalhães, Andreia Patrícia Alves Grainha, Tânia Raquel R. Sousa, Ana Margarida França, Ângela Maria Oliveira Sousa Cerca, Nuno Pereira, Maria Olívia |
Palavras-chave: | Pseudomonas aeruginosa Staphylococcus aureus Biofilm Interspecies interaction Viable but non-culturable cells Cystic fibrosis |
Data: | Set-2021 |
Editora: | Wiley-Blackwell |
Revista: | Environmental Microbiology |
Citação: | Magalhães, Andreia P.; Grainha, Tânia; Sousa, Ana Margarida; França, Angela; Cerca, Nuno; Pereira, Maria Olívia, Viable but non-cultivable state: a strategy for Staphylococcus aureus survivable in dual-species biofilms with Pseudomonas aeruginosa?. Environmental Microbiology, 23(9), 5639-5649, 2021 |
Resumo(s): | Summary Pseudomonas aeruginosa and Staphylococcus aureus are two of the most prevalent respiratory pathogens in cystic fibrosis (CF) patients. Both organisms often cause chronic and recalcitrant infections, in large part due to their ability to form biofilms, being these mixed-species infections correlated with poor clinical outcomes. In this study, the hypothesis that S. aureus adopts phenotypes allowing its coexistence with P. aeruginosa during biofilm growth was put forward. We noticed that S. aureus undergoes a viable but non-cultivable (VBNC) state in the dominated P. aeruginosa dual-species consortia, whatsoever the strains used to form the biofilms. Moreover, an increased expression of genes associated with S. aureus virulence was detected suggesting that the phenotypic switching to VBNC state might account for S. aureus pathogenicity and, in turn, influence the clinical outcome of the mixed-species infection. Thus, P. aeruginosa seems to induce both phenotypic and transcriptomic changes in S. aureus, helping its survival and co-existence in the dual-species biofilms. Overall, our findings illustrate how interspecies interactions can modulate bacterial virulence in vitro, contributing to a better understanding of the behaviour of P. aeruginosa-S. aureus dual-species biofilms. This article is protected by copyright. All rights reserved. |
Tipo: | Artigo |
Descrição: | Accepted Article |
URI: | https://hdl.handle.net/1822/74215 |
DOI: | 10.1111/1462-2920.15734 |
ISSN: | 1462-2912 |
Versão da editora: | https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.15734 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54593_1.pdf Acesso restrito! | 1,21 MB | Adobe PDF | Ver/Abrir |