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https://hdl.handle.net/1822/73521
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pontes, Rita | por |
dc.contributor.author | Romani, Aloia Perez | por |
dc.contributor.author | Michelin, Michele | por |
dc.contributor.author | Domingues, Lucília | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Nunes, João | por |
dc.date.accessioned | 2021-07-05T10:41:15Z | - |
dc.date.issued | 2021-10-15 | - |
dc.date.submitted | 2020-12 | - |
dc.identifier.citation | Pontes, Rita; Romaní, Aloia; Michelin, Michele; Domingues, Lucília; Teixeira, José A.; Nunes, João, L-lactic acid production from multi-supply autohydrolyzed economically unexploited lignocellulosic biomass. Industrial Crops and Products, 170(113775), 2021 | por |
dc.identifier.issn | 0926-6690 | por |
dc.identifier.uri | https://hdl.handle.net/1822/73521 | - |
dc.description.abstract | The present paradigm of a new concept of biomass conversion system has been focused on the development of a multi-supply biorefinery concept towards a circular and sustainable bioeconomy. This work was focused on evaluating a mixture of six lignocellulosic biomass types, from forest ecosystems and biological resources from marginal land, in order to produce lactic acid (L-LA) by Lactobacillus rhamnosus within a multi-supply biorefinery scheme. The mixture of lignocellulosic biomass was submitted to autohydrolysis pretreatment under non-isothermal regime at 226 °C (corresponding to a severity of 4.15) yielding a glucan recovery of 93 % in the solid phase. Two different strategies were assayed for L-LA production, namely separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). The glucose to LA yield obtained for both assays was 1 g/g, although the volumetric productivity of SSF (2.5 g/Lh) was higher than SHF (0.8 g/Lh). Therefore, the SSF process was optimized through a factorial design to evaluate the effect of the independent variables, solids load (SL) and enzyme-substrate ratio (ESR), on LA production. The maximum concentration of LA was obtained using the highest solids load (16 %) and with the highest ESR (54 FPU/g). Finally, L-LA was produced using in a bioreactor under the optimized conditions from experimental design, obtaining 61.74 g/L of LA at 44 h, with an optical purity of 98 % and a LA yield of 0.97 g/g. This study showed the potential of multi-supply unexploited lignocellulosic biomass as a raw material to LA fermentation. | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of SFRH/BDE/109316/2015 unit, UIDB/04469/2020 unit, BLC3 Evolution Lda., COMPETE 2020 [3iBioeconomia: POCI-01-0246-FEDER-026758], COMPETE 2020 [POCI-01-0145-FEDER-006684], BioTecNorte operation [NORTE-01-0145-FEDER-000004] funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and FEDER through COMPETE and of National Funds through FCT grants FCOMP-01-0124-FEDER-028359 [PTDC/BIA-MIC/2779/2012] and UID/NEU/04539/2013 | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | restrictedAccess | por |
dc.subject | Lignocellulosic biomass mixture | por |
dc.subject | Autohydrolysis | por |
dc.subject | Simultaneous saccharification and fermentation | por |
dc.subject | Lactic acid | por |
dc.subject | Biorefinery | por |
dc.subject | Lactobacillus rhamnosus | por |
dc.title | L-lactic acid production from multi-supply autohydrolyzed economically unexploited lignocellulosic biomass | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.elsevier.com/locate/issn/09266690 | por |
dc.comments | CEB54514 | por |
oaire.citationIssue | 113775 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 170 | por |
dc.date.updated | 2021-07-03T10:54:46Z | - |
dc.identifier.doi | 10.1016/j.indcrop.2021.113775 | por |
dc.date.embargo | 10000-01-01 | - |
dc.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Industrial Crops and Products | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_54514_1.pdf Acesso restrito! | 3,24 MB | Adobe PDF | Ver/Abrir |