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https://hdl.handle.net/1822/73521
Título: | L-lactic acid production from multi-supply autohydrolyzed economically unexploited lignocellulosic biomass |
Autor(es): | Pontes, Rita Romani, Aloia Perez Michelin, Michele Domingues, Lucília Teixeira, J. A. Nunes, João |
Palavras-chave: | Lignocellulosic biomass mixture Autohydrolysis Simultaneous saccharification and fermentation Lactic acid Biorefinery Lactobacillus rhamnosus |
Data: | 15-Out-2021 |
Editora: | Elsevier 1 |
Revista: | Industrial Crops and Products |
Citação: | Pontes, Rita; Romaní, Aloia; Michelin, Michele; Domingues, Lucília; Teixeira, José A.; Nunes, João, L-lactic acid production from multi-supply autohydrolyzed economically unexploited lignocellulosic biomass. Industrial Crops and Products, 170(113775), 2021 |
Resumo(s): | The present paradigm of a new concept of biomass conversion system has been focused on the development of a multi-supply biorefinery concept towards a circular and sustainable bioeconomy. This work was focused on evaluating a mixture of six lignocellulosic biomass types, from forest ecosystems and biological resources from marginal land, in order to produce lactic acid (L-LA) by Lactobacillus rhamnosus within a multi-supply biorefinery scheme. The mixture of lignocellulosic biomass was submitted to autohydrolysis pretreatment under non-isothermal regime at 226 °C (corresponding to a severity of 4.15) yielding a glucan recovery of 93 % in the solid phase. Two different strategies were assayed for L-LA production, namely separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). The glucose to LA yield obtained for both assays was 1 g/g, although the volumetric productivity of SSF (2.5 g/Lh) was higher than SHF (0.8 g/Lh). Therefore, the SSF process was optimized through a factorial design to evaluate the effect of the independent variables, solids load (SL) and enzyme-substrate ratio (ESR), on LA production. The maximum concentration of LA was obtained using the highest solids load (16 %) and with the highest ESR (54 FPU/g). Finally, L-LA was produced using in a bioreactor under the optimized conditions from experimental design, obtaining 61.74 g/L of LA at 44 h, with an optical purity of 98 % and a LA yield of 0.97 g/g. This study showed the potential of multi-supply unexploited lignocellulosic biomass as a raw material to LA fermentation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/73521 |
DOI: | 10.1016/j.indcrop.2021.113775 |
ISSN: | 0926-6690 |
Versão da editora: | http://www.elsevier.com/locate/issn/09266690 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54514_1.pdf Acesso restrito! | 3,24 MB | Adobe PDF | Ver/Abrir |