Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/73456
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, C. S. | por |
dc.contributor.author | Pinto, R. D. | por |
dc.contributor.author | Amorim, S. | por |
dc.contributor.author | Pires, R. A. | por |
dc.contributor.author | Correia-Neves, M. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Alves, N. L. | por |
dc.contributor.author | Martins, A. | por |
dc.contributor.author | Neves, N. M. | por |
dc.date.accessioned | 2021-06-25T11:15:48Z | - |
dc.date.issued | 2020-11 | - |
dc.date.submitted | 2021-06 | - |
dc.identifier.citation | Silva, C. S., Pinto, R. D., Amorim, S., Pires, R. A., et. al (2020). Fibronectin-Functionalized Fibrous Meshes as a Substrate to Support Cultures of Thymic Epithelial Cells. Biomacromolecules, 21(12), 4771-4780 | por |
dc.identifier.issn | 1525-7797 | por |
dc.identifier.uri | https://hdl.handle.net/1822/73456 | - |
dc.description.abstract | Thymic epithelial cells (TECs) are the main regulators of TÂ lymphocyte development and selection, requiring a three-dimensional (3D) environment to properly perform these biological functions. The aim of this work was to develop a 3D culture substrate that allows the survival and proliferation of TECs. Thus, electrospun fibrous meshes (eFMs) were functionalized with fibronectin, one of the major extracellular matrix (ECM) proteins of the thymus. For that, highly porous eFMs were activated using oxygen plasma treatment followed by amine insertion, which allows the immobilization of fibronectin through EDC/NHS chemistry. The medullary TECs presented increased proliferation, viability, and protein synthesis when cultured on fibronectin-functionalized eFMs (FN-eFMs). These cells showed a spread morphology, with increased migration toward the inner layers of FN-eFMs and the production of thymic ECM proteins, such as collagen type IV and laminin. These results suggest that FN-eFMs are an effective substrate for supporting thymic cell cultures. | por |
dc.description.sponsorship | Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, for the PhD grant of C.S.S. (UMINHO/BD/33/2016; NORTE-08-5369- FSE-000012) and the project FRONthera (NORTE-01-0145- FEDER-0000232). The Portuguese Foundation for Science and Technology (FCT) for the IF grant of A.M. (IF/00376/ 2014) and the project SPARTAN (PTDC/CTM-BIO/4388/ 2014) is also acknowledged. This work was also supported by a starting grant of N.L.A. from the European Research Council (ERC) under the project 637843 | por |
dc.language.iso | eng | por |
dc.publisher | ACS Publications | por |
dc.rights | closedAccess | por |
dc.subject | Electrospun fibrous meshes | por |
dc.subject | Fibronectin | por |
dc.subject | Functionalization | por |
dc.subject | Thymic epithelial cells | por |
dc.subject | Thymus bioengineering | por |
dc.title | Fibronectin-Functionalized fibrous meshes as a substrate to support cultures of thymic epithelial cells | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://pubs.acs.org/doi/abs/10.1021/acs.biomac.0c00933 | por |
dc.comments | http://3bs.uminho.pt/node/20542 | por |
oaire.citationStartPage | 4771 | por |
oaire.citationEndPage | 4780 | por |
oaire.citationIssue | 12 | por |
oaire.citationVolume | 21 | por |
dc.date.updated | 2021-06-25T08:59:05Z | - |
dc.identifier.eissn | 1526-4602 | por |
dc.identifier.doi | 10.1021/acs.biomac.0c00933 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 33238090 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomacromolecules | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20542-acsbiomac0c00933.pdf Acesso restrito! | 1,12 MB | Adobe PDF | Ver/Abrir |