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https://hdl.handle.net/1822/73223
Título: | New insights into the immunoproteome of B. cenocepacia J2315 using serum samples from cystic fibrosis patients |
Autor(es): | Sousa, Sílvia A Soares-Castro, Pedro Seixas, António M M Feliciano, Joana R Balugas, Bernardo Barreto, Celeste Pereira, Luísa Santos, P. M. Leitão, Jorge H |
Palavras-chave: | Burkholderia cenocepacia Child Computational Biology Cystic Fibrosis Electrophoresis, Gel, Two-Dimensional Humans Male Proteome Burkholderia cepacia complex Immunoproteome Vaccine Immunodiagnostics Serum samples |
Data: | 2020 |
Editora: | Elsevier 1 |
Revista: | New Biotechnology |
Citação: | Sousa, S. A., Soares-Castro, P., Seixas, A. M. M., Feliciano, J. R., Balugas, B., Barreto, C., . . . Leitão, J. H. (2020). New insights into the immunoproteome of B. cenocepacia J2315 using serum samples from cystic fibrosis patients. New Biotechnology, 54, 62-70. doi: https://doi.org/10.1016/j.nbt.2019.08.006 |
Resumo(s): | Bacteria of the Burkholderia cepacia complex (Bcc) are ubiquitous multidrug resistant organisms and opportunistic pathogens capable of causing life threatening lung infections among cystic fibrosis (CF) patients. No effective therapies are currently available to eradicate Bcc bacteria from CF patients, as these organisms are inherently resistant to the majority of clinically available antimicrobials. An immunoproteomics approach was used to identify Bcc proteins that stimulate the humoral immune response of the CF host, using bacterial cells grown under conditions mimicking the CF lung environment and serum samples from CF patients with a clinical record of Bcc infection. 24 proteins of the Bcc strain B. cenocepacia J2315 were identified as immunoreactive, 19 here reported as immunogenic for the first time. Ten proteins were predicted as extracytoplasmic, 9 of them being conserved in Bcc genomes. The immunogenic Bcc extracytoplasmic proteins are potential targets for development of novel therapeutic strategies and diagnostic tools to protect patients against the onset of chronic Bcc lung infections. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/73223 |
DOI: | 10.1016/j.nbt.2019.08.006 |
ISSN: | 1871-6784 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S1871678418316960 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S1871678418316960-main.pdf Acesso restrito! | 2,52 MB | Adobe PDF | Ver/Abrir |