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https://hdl.handle.net/1822/71185
Título: | Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues |
Autor(es): | Scarcella, Ana Sílvia de Almeida Pasin, Thiago Machado Oliveira, Tássio Brito de Lucas, Rosymar Coutinho de Ferreira-Nozawa, Monica Stropa Freitas, Emanuelle Neiverth de Vici, Ana Claudia Buckeridge, Marcos Silveira Michelin, Michele Polizeli, Maria de Lourdes Teixeira de Moraes |
Palavras-chave: | Enzymatic cocktail Sugarcane biomass Energy cane Co-cultivation Mycothermus thermophilus Trichoderma reesei |
Data: | Jul-2021 |
Editora: | Elsevier 1 |
Revista: | Energy |
Citação: | Scarcella, Ana Sílvia de Almeida; Pasin, Thiago Machado; Oliveira, Tássio Brito de; Lucas, Rosymar Coutinho de; Ferreira-Nozawa, Monica Stropa; Freitas, Emanuelle Neiverth de; Vici, Ana Claudia; Buckeridge, Marcos Silveira; Michelin, Michele; Polizeli, Maria de Lourdes Teixeira de Moraes, Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues. Energy, 226(120360), 2021 |
Resumo(s): | Lignocellulosic biomasses are promising sources for the obtaining of clean energy through their bioconversion into ethanol. Their saccharification involves a multi-enzymatic system. Here, we evaluated the hydrolysis of a mixture of sugarcane bagasse varieties (SCB), Energy cane fiber (EC), and sugarcane bagasse from the SP80-3280 variety, all in natura, using fungal enzymatic extracts obtained from Mycothermus thermophilus and Trichoderma reesei RP698 cultures supplemented with various agro-industrial residues. The enzymatic extracts from both fungi, when grown in a corn cob and corn straw, led to the highest sugarcane hydrolysis. For M. thermophilus, the reducing sugars released (mg/mL) were 1.21 ± 0.12, 1.25 ± 0.14, and 0.98 ± 0.05 for SCB, EC and SP80-3280, respectively; for T. reesei, the reducing sugars were 0.84 ± 0.08, 0.89 ± 0.06 and 0.68 ± 0.03 for SCB, SP80-3280, and EC, respectively. The cocktail obtained from the co-cultivation of these fungi in corn straw at 35 °C showed the best hydrolysis results, the release of sugars (mg/mL) was 1.31 ± 0.06 (SCB), 2.18 ± 0.08 (EC) and 1.67 ± 0.09 (SP80-3280). Scanning electron microscopy and thermogravimetric analysis confirmed changes in the fiber structures after enzymatic hydrolysis. Thus, these fungi were shown to be promising for an enzymatic cocktail design and sugarcane biomass saccharification. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/71185 |
DOI: | 10.1016/j.energy.2021.120360 |
ISSN: | 0360-5442 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0360544221006095 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54357_1.pdf Acesso restrito! | 1,71 MB | Adobe PDF | Ver/Abrir |