Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/71169
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Pinheiro, T. | por |
dc.contributor.author | Lip, Ka Ying Florence | por |
dc.contributor.author | García-Ríos, Estéfani | por |
dc.contributor.author | Querol, Amparo | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | van Gulik, Walter | por |
dc.contributor.author | Guillamón, José Manuel | por |
dc.contributor.author | Domingues, Lucília | por |
dc.date.accessioned | 2021-04-01T08:38:36Z | - |
dc.date.available | 2021-04-01T08:38:36Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Pinheiro, T.; Lip, Ka Ying Florence; García-Ríos, Estéfani; Querol, Amparo; Teixeira, José A.; van Gulik, Walter; Guillamón, José Manuel; Domingues, Lucília, Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions. Scientific Reports, 10(22329), 2020 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/71169 | - |
dc.description.abstract | Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, Ethanol Red, grown at sub- and supra-optimal temperatures under chemostat conditions. We employed anaerobic conditions, mimicking the industrial processes. The proteomic profile of these strains in all conditions was performed by sequential window acquisition of all theoretical spectra-mass spectrometry (SWATH-MS), allowing the quantification of 997 proteins, data available via ProteomeXchange (PXD016567). Our analysis demonstrated that temperature responses differ between the strains; however, we also found some common responsive proteins, revealing that the response to temperature involves general stress and specific mechanisms. Overall, sub-optimal temperature conditions involved a higher remodeling of the proteome. The proteomic data evidenced that the cold response involves strong repression of translation-related proteins as well as induction of amino acid metabolism, together with components related to protein folding and degradation while, the high temperature response mainly recruits amino acid metabolism. Our study provides a global and thorough insight into how growth temperature affects the yeast proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance. | por |
dc.description.sponsorship | Financial support is acknowledged to Project ERA-IB “YeastTempTation” (ERA-IB-2-6/0001/2014), and FCT for the strategic funding of UIDB/04469/2020 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), and BioTecNorte operation (NORTE-01-0145-FEDER-000004). Lallemand Ibéria, SA is acknowledged for the supply of yeast strains. Te proteomic analysis was carried out in the SCSIE University of Valencia Proteomics Unit, a member of the ISCIII ProteoRed Proteomics Platform. Authors thank to Luz Valero, researcher of the Proteomic unit, for her valuable support. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Publishing Group | por |
dc.relation | UIDB/04469/2020 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.title | Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.nature.com/srep/index.html | por |
dc.comments | CEB54073 | por |
oaire.citationIssue | 22329 | por |
oaire.citationVolume | 10 | por |
dc.date.updated | 2021-02-15T17:26:31Z | - |
dc.identifier.doi | 10.1038/s41598-020-77846-w | por |
dc.identifier.pmid | 33339840 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54073_1.pdf | 2,16 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons