Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/71048
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Campo DC | Valor | Idioma |
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dc.contributor.author | Oliveira, Alexandre Rafael Machado | por |
dc.contributor.author | Rodrigues, Joana Lúcia Lima Correia | por |
dc.contributor.author | Ferreira, Eugénio C. | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.contributor.author | Dias, Oscar | por |
dc.date.accessioned | 2021-03-28T21:47:01Z | - |
dc.date.available | 2021-03-28T21:47:01Z | - |
dc.date.issued | 2021-03-08 | - |
dc.identifier.citation | Oliveira, Alexandre; Rodrigues, Joana L.; Ferreira, Eugénio C.; Rodrigues, Lígia R.; Dias, Oscar, A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli. PLoS Computational Biology, 17(3), e1008704, 2021. DOI: 10.1371/journal.pcbi.1008704 | por |
dc.identifier.issn | 1553-734X | por |
dc.identifier.uri | https://hdl.handle.net/1822/71048 | - |
dc.description.abstract | Acrylic acid is an economically important chemical compound due to its high market value. Nevertheless, the majority of acrylic acid consumed worldwide its produced from petroleum derivatives by a purely chemical process, which is not only expensive, but it also contributes towards environment deterioration. Hence, justifying the current need for sustainable novel production methods that allow higher profit margins. Ideally, to minimise production cost, the pathway should consist in the direct bio-based production from microbial feedstocks, such as Escherichia coli, but the current yields achieved are still too low to compete with conventional method. In this work, even though the glycerol pathway presented higher yields, we identified the malonyl-CoA route, when using glucose as carbon source, as having the most potential for industrial-scale production, since it is cheaper to implement. Furthermore, we also identified potential optimisation targets for all the tested pathways, that can help the bio-based method to compete with the conventional process. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology(FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. This article is also a result of the project 22231/01/SAICT/2016: “Biodata.pt – Infraestrutura Portuguesa de Dados Biolo´gicos”, by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Alexandre Oliveira holds a doctoral fellowship (2020.10205.BD) provided by the FCT. Oscar Dias acknowledge FCT for the Assistant Research contract obtained under CEEC Individual 2018. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | por |
dc.language.iso | eng | por |
dc.publisher | Public Library of Science | por |
dc.relation | UIDB/04469/2020 | por |
dc.relation | 22231/01/SAICT/2016 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.title | A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://journals.plos.org/ploscompbiol/ | por |
dc.comments | CEB54285 | por |
oaire.citationIssue | 3 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationVolume | 17 | por |
dc.date.updated | 2021-03-28T16:28:22Z | - |
dc.identifier.doi | 10.1371/journal.pcbi.1008704 | por |
dc.identifier.pmid | 33684125 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | PLoS Computational Biology | por |
dc.identifier.articlenumber | e1008704 | - |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54285_1.pdf | 2,72 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons