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https://hdl.handle.net/1822/71039
Título: | Co-cultivation of Thermoanaerobacter strains with a methanogenic partner enhances glycerol conversion |
Autor(es): | Magalhães, Carla Isabel Pereira Ribeiro, Joaquim Alfredo França Martins Guedes, Ana P. Arantes, Ana Luísa Sousa, Diana Zita Machado Stams, Alfons Johannes Maria Alves, M. M. Cavaleiro, Ana Júlia |
Data: | Jul-2020 |
Editora: | Wiley-Blackwell |
Revista: | Microbial Biotechnology |
Citação: | Magalhães, C. P.; Ribeiro, J.; Guedes, Ana P.; Arantes, Ana Luísa; Sousa, Diana Z.; Stams, A. J. M.; Alves, M. Madalena; Cavaleiro, Ana Júlia, Co-cultivation of Thermoanaerobacter strains with a methanogenic partner enhances glycerol conversion. Microbial Biotechnology, 13(4), 962-973, 2020 |
Resumo(s): | Glycerolrich waste streams produced by the biodiesel, bioethanol and oleochemical industries can be treated and valorized by anaerobic microbial communities to produce methane. As current knowledge of the microorganisms involved in thermophilic glycerol conversion to methane is scarce, thermophilic glyceroldegrading methanogenic communities were enriched. A coculture of Thermoanaerobacter and Methanothermobacter species was obtained, pointing to a nonobligately syntrophic glycerol degradation. This hypothesis was further studied by incubating Thermoanaerobacter brockii subsp. finnii and T. wiegelii with glycerol (10 mM) in pure culture and with different hydrogenotrophic methanogens. The presence of the methanogen accelerated glycerol fermentation by the two Thermoanaerobacter strains up to 3.3 mM day1, corresponding to 12 times higher volumetric glycerol depletion rates in the methanogenic cocultures than in the pure bacterial cultures. The catabolic pathways of glycerol conversion were identified by genome analysis of the two Thermoanaerobacter strains. NADH and reduced ferredoxin formed in the pathway are linked to proton reduction, which becomes thermodynamically favourable when the hydrogen partial pressure is kept low by the hydrogenotrophic methanogenic partner. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/71039 |
DOI: | 10.1111/1751-7915.13506 |
ISSN: | 1751-7915 |
Versão da editora: | https://onlinelibrary.wiley.com/journal/17517915 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53589_1.pdf | 754,39 kB | Adobe PDF | Ver/Abrir |