Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/68463
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Mendes, Bárbara B. | por |
dc.contributor.author | Daly, Andrew C. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Domingues, Rui Miguel Andrade | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.contributor.author | Burdick, Jason A. | por |
dc.date.accessioned | 2020-12-09T18:32:53Z | - |
dc.date.available | 2021-11-01T07:00:54Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2020-11 | - |
dc.identifier.citation | Mendes B. B., Daly A. C., Reis R. L., Domingues R. M. A., Gomes M. E., Burdick J. A. Injectable hyaluronic acid and platelet lysate-derived granular hydrogels for biomedical applications, Acta Biomaterialia, pp. in press, doi:10.1016/j.actbio.2020.10.040, 2021. | por |
dc.identifier.issn | 1742-7061 | por |
dc.identifier.uri | https://hdl.handle.net/1822/68463 | - |
dc.description.abstract | Towards the repair of damaged tissues, numerous scaffolds have been fabricated to recreate the complex extracellular matrix (ECM) environment to support desired cell behaviors; however, it is often challenging to design scaffolds with the requisite cell-anchorage sites, mechanical stability, and tailorable physicochemical properties necessary for many applications. To address this and to improve on the properties of hyaluronic acid (HA) hydrogels, we combined photocrosslinkable norbornene-modified HA (NorHA) with human platelet lysate (PL). These PL-NorHA hybrid hydrogels supported the adhesion of cells when compared to NorHA hydrogels without PL, exhibited tailorable physicochemical properties based on the concentration of individual components, and released proteins over time. Using microfluidic techniques with on-chip mixing of NorHA and PL and subsequent photocrosslinking, spherical PL-NorHA microgels with a hierarchical fibrillar network were fabricated that exhibited the sustained delivery of PL proteins. Microgels could be jammed into granular hydrogels that exhibited shear-thinning and self-healing properties, enabling ejection from syringes and the fabrication of stable 3D constructs with 3D printing. Again, the inclusion of PL enhanced cellular interactions with the microgel structures. Overall, the combination of biomolecules and fibrin self-assembly arising from the enriched milieu of PL-derived proteins improved the bioactivity of HA-based hydrogels, enabling the formation of dynamic systems with modular design. The granular systems can be engineered to meet the complex demands of functional tissue repair using versatile processing techniques, such as with 3D printing. | por |
dc.description.sponsorship | The authors acknowledge the financial support from FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior), the Fundo Social Europeu através do Programa Operacional do Capital Humano (FSE/POCH) in the framework of PhD grant PD/59/2013 – PD/BD/113807/2015 (BBM) and the National Science Foundation MRSEC (DMR-1720530). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Bioactive | por |
dc.subject | Hyaluronic acid | por |
dc.subject | microgels | por |
dc.subject | Platelet lysate | por |
dc.title | Injectable hyaluronic acid and platelet lysate-derived granular hydrogels for biomedical applications | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://doi.org/10.1016/j.actbio.2020.10.040 | por |
dc.comments | http://3bs.uminho.pt/node/20427 | por |
oaire.citationStartPage | 101 | por |
oaire.citationEndPage | 113 | por |
oaire.citationVolume | 119 | por |
dc.date.updated | 2020-12-03T17:35:28Z | - |
dc.identifier.doi | 10.1016/j.actbio.2020.10.040 | por |
dc.identifier.pmid | 33130309 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Acta Biomaterialia | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20427-Mendes2020_Injectable hyaluronic acid and platelet lysate-derived granular hydrogels for biomedical applications.pdf | 4,25 MB | Adobe PDF | Ver/Abrir |