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https://hdl.handle.net/1822/68040
Título: | In vitro temporal HIF‐mediated deposition of osteochondrogenic matrix governed by hypoxia and osteogenic factors synergy |
Autor(es): | Calejo, I. Costa-Almeida, R. Reis, R. L. Gomes, Manuela E. |
Palavras-chave: | Cell modulation Enthesis Hedgehog signaling Hypoxia-inducible factors Osteogenic supplementation Hypoxia‐ inducible factors Hypoxia‐ |
Data: | Nov-2020 |
Editora: | Wiley |
Revista: | Journal of Cellular Physiology |
Citação: | Calejo I., Costa-Almeida R., Reis R. L., Gomes M. E. In vitro temporal HIF‐mediated deposition of osteochondrogenic matrix governed by hypoxia and osteogenic factors synergy, Journal Of Cellular Physiology, doi:10.1002/jcp.30138, 2020 |
Resumo(s): | Musculoskeletal interfaces are naturally hypoxic. An understanding of key interactions occurring between different cell populations and their environment is critical for native tissue recapitulation. Here, an enthesis coculture model (preosteoblasts and tendon cells) was used to understand the influence of hypoxia (5% O2) and osteogenic medium (OM) supplementation in cellsâ phenotype modulation. In single cultures, preosteoblasts were found to undergo osteogenic impairment, while tendon cells underwent a maturation process through extracellular matrix (ECM) rescue. When in coâ culture, hypoxia and osteoinduction promoted a temporal chondro/osteogenic pathway activation, as observed by an early deposition of cartilaginous ECM associated with HIF1A stabilization and RUNX2 activation, and later hypertrophic differentiation resulting from HIF2A translocation and SOX9 activation. Moreover, the presence of OM under hypoxia was shown to influence the extracellular ROS/HIF1A interplay. Overall, this study revealed a link between biochemical factors and cellâ cell crosstalk, providing a molecular framework for hypoxic control and modulation of cellsâ fate toward enthesisâ like phenotypes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/68040 |
DOI: | 10.1002/jcp.30138 |
ISSN: | 0021-9541 |
e-ISSN: | 1097-4652 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/jcp.30138 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20411-Calejo et al_2020_Journal_of_cellular_physiology.pdf Acesso restrito! | 14,47 MB | Adobe PDF | Ver/Abrir |