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https://hdl.handle.net/1822/67782
Título: | Aberrant translation of cytochrome c oxidase subunit 1 mRNA species in the absence of Mss51p in the yeast Saccharomyces cerevisiae |
Autor(es): | Zambrano, Andrea Fontanesi, Flavia Solans, Asun de Oliveira, Rodrigo Leite Fox, Thomas D. Tzagoloff, Alexander Barrientos, Antoni |
Palavras-chave: | 5' Untranslated Regions Chloramphenicol Cytochromes c1 Membrane Proteins Mitochondrial Proteins Mutation Nuclear Proteins Peptide Initiation Factors Peptides Protein Biosynthesis RNA, Messenger Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal |
Data: | Fev-2007 |
Editora: | American Society for Cell Biology |
Revista: | Molecular Biology of the Cell |
Citação: | ZAMBRANO, Andrea et al. Aberrant translation of cytochrome c oxidase subunit 1 mRNA species in the absence of Mss51p in the yeast Saccharomyces cerevisiae. Molecular biology of the cell, v. 18, n. 2, p. 523-535, 2007 |
Resumo(s): | Expression of yeast mitochondrial genes depends on specific translational activators acting on the 5'-untranslated region of their target mRNAs. Mss51p is a translational factor for cytochrome c oxidase subunit 1 (COX1) mRNA and a key player in down-regulating Cox1p expression when subunits with which it normally interacts are not available. Mss51p probably acts on the 5'-untranslated region of COX1 mRNA to initiate translation and on the coding sequence itself to facilitate elongation. Mss51p binds newly synthesized Cox1p, an interaction that could be necessary for translation. To gain insight into the different roles of Mss51p on Cox1p biogenesis, we have analyzed the properties of a new mitochondrial protein, mp15, which is synthesized in mss51 mutants and in cytochrome oxidase mutants in which Cox1p translation is suppressed. The mp15 polypeptide is not detected in cox14 mutants that express Cox1p normally. We show that mp15 is a truncated translation product of COX1 mRNA whose synthesis requires the COX1 mRNA-specific translational activator Pet309p. These results support a key role for Mss51p in translationally regulating Cox1p synthesis by the status of cytochrome oxidase assembly. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67782 |
DOI: | 10.1091/mbc.e06-09-0803 |
ISSN: | 1059-1524 |
e-ISSN: | 1939-4586 |
Versão da editora: | https://www.molbiolcell.org/doi/full/10.1091/mbc.e06-09-0803 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Zambrano-2006-Aberrant-translation-of-cytochrome-.pdf | 2,32 MB | Adobe PDF | Ver/Abrir |