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https://hdl.handle.net/1822/67556
Título: | Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
Autor(es): | Sheeba, Caroline J. Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
Palavras-chave: | Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick |
Data: | 2010 |
Editora: | University of the Basque Country (UPV/EHU) |
Revista: | International Journal of Developmental Biology |
Resumo(s): | Specific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4, 8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67556 |
DOI: | 10.1387/ijdb.092887cs |
ISSN: | 0214-6282 |
e-ISSN: | 1696-3547 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Sheeba-2010-Comprehensive-analysis-of-fibroblas.pdf | 4,91 MB | Adobe PDF | Ver/Abrir |