Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/67465
Título: | Effective cytocompatible nanovectors based on serine-derived gemini surfactants and monoolein for small interfering RNA delivery |
Autor(es): | Costa, Catarina Oliveira, Isabel S. Silva, João P. N. Silva, Sandra G. Botelho, Cláudia M. Vale, M. C. Real Oliveira, M. Elisabete C.D. Gomes, Andreia C Marques, Eduardo F. |
Palavras-chave: | Gemini surfactant Serine siRNA-delivery Lipoplex properties Monoolein helper lipid Transfection efficiency |
Data: | 2021 |
Editora: | Elsevier 1 |
Revista: | Journal of Colloid and Interface Science |
Citação: | Costa, Catarina; Oliveira, Isabel S.; Silva, João P. N.; Silva, Sandra G.; Botelho, Cláudia M.; Vale, M. C.; Oliveira, Maria Elisabete C. D. R.; Gomes, Andreia C.; Marques, Eduardo F., Effective cytocompatible nanovectors based on serine-derived gemini surfactants and monoolein for small interfering RNA delivery. Journal of Colloid and Interface Science, 584, 34-44, 2021 |
Resumo(s): | Non-viral gene therapy based on gene silencing with small interfering RNA (siRNA) has attracted great interest over recent years. Among various types of cationic complexation agents, amino acid-based surfactants have been recently explored for nucleic acid delivery due to their low toxicity and high biocompatibility. Monoolein (MO), in turn, has been used as helper lipid in liposomal systems due to its ability to form inverted nonbilayer structures that enhance fusogenicity, thus contributing to higher transfection efficiency. In this work, we focused on the development of nanovectors for siRNA delivery based on three gemini amino acid-based surfactants derived from serine (12Ser)2N12, amine derivative; (12Ser)2COO12, ester derivative; and (12Ser)2CON12, amide derivative individually combined with MO as helper lipid. The inclusion of MO in the cationic surfactant system influences the morphology and size of the mixed aggregates. Furthermore, the gemini surfactant:MO systems showed the ability to efficiently complex siRNA, forming stable lipoplexes, in some cases clearly depending on the MO content, without inducing significant levels of cytotoxicity. High levels of gene silencing were achieved in comparison with a commercially available standard indicating that these gemini:MO systems are promising candidates as lipofection vectors for RNA interference (RNAi)-based therapies. |
Tipo: | Artigo |
Descrição: | Supplementary data to this article can be found online athttps://doi.org/10.1016/j.jcis.2020.09.077. |
URI: | https://hdl.handle.net/1822/67465 |
DOI: | 10.1016/j.jcis.2020.09.077 |
ISSN: | 0021-9797 |
e-ISSN: | 1095-7103 |
Versão da editora: | https://www.sciencedirect.com/science/article/abs/pii/S0021979720312662 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series PHYSICS OF QUANTUM MATERIALS AND BIONANOSTRUCTURES (2018 - ...) CBMA - Artigos/Papers CDF - FAMO - Artigos/Papers (with refereeing) DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53908_1.pdf | 2,94 MB | Adobe PDF | Ver/Abrir |