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https://hdl.handle.net/1822/66365
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Campo DC | Valor | Idioma |
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dc.contributor.author | Matos, A. M. | por |
dc.contributor.author | Gonçalves, A. I. | por |
dc.contributor.author | Rodrigues, M. T. | por |
dc.contributor.author | Miranda, M. S. | por |
dc.contributor.author | Haj, A. E. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.date.accessioned | 2020-08-06T17:33:26Z | - |
dc.date.available | 2020-08-06T17:33:26Z | - |
dc.date.issued | 2020-09 | - |
dc.date.submitted | 2020-08 | - |
dc.identifier.citation | Matos A. M., Gonçalves A. I., Rodrigues M. T., Miranda M. S., Haj A. E., Reis R. L., Gomes M. E. Remote triggering of TGF-β/Smad2/3 signaling in human adipose stem cells laden on magnetic scaffolds synergistically promotes tenogenic commitment, Acta Biomaterialia, Vol. 113, pp. 488-500, doi:10.1016/j.actbio.2020.07.009, 2020 | por |
dc.identifier.issn | 1742-7061 | por |
dc.identifier.uri | https://hdl.handle.net/1822/66365 | - |
dc.description.abstract | Injuries affecting load bearing tendon tissues are a significant clinical burden and efficient treatments are still unmet. Tackling tendon regeneration, tissue engineering strategies aim to develop functional substitutes that recreate native tendon milieu. Tendon mimetic scaffolds capable of remote magnetic responsiveness and functionalized magnetic nanoparticles (MNPs) targeting cellular mechanosensitive receptors are potential instructive tools to mediate mechanotransduction in guiding tenogenic responses. In this work, we combine magnetically responsive scaffolds and targeted Activin A type II receptor in human adipose stem cells (hASCs), under alternating magnetic field (AMF), to synergistically facilitate external control over signal transduction. The combination of remote triggering TGF-β/Smad2/3 using MNPs tagged hASCs, through magnetically actuated scaffolds, stimulates overall expression of tendon related genes and the deposition of tendon related proteins, in comparison to non-stimulated conditions. Moreover, the phosphorylation of Smad2/3 proteins and their nuclear co-localization was also more evident. Overall, biophysical stimuli resulting from magnetic scaffolds and magnetically triggered cells under AMF stimulation modulate the mechanosensing response of hASCs towards tenogenesis, holding therapeutic promise. | por |
dc.description.sponsorship | Authors acknowledge the project “Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marine-derived biomaterials and stem cells”, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and the FCT Project MagTT PTDC/CTM-CTM/29930/2017 (POCI-01-0145-FEDER-29930). Authors acknowledge the HORIZON 2020 for the Achilles Twinning Project No. 810850. Authors also thank the European Research Council COG MagTendon No. 772817 and the ADG DYNACEUTICS No. 789119. Prof. Bernardo Almeida from Physics Department, University of Minho, is also acknowledged for assisting in the magnetic system assembling. Authors also acknowledge the INL - International Iberian Nanotechnology Laboratory (Braga, Portugal) for the magnetization analysis. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | PTDC/CTM-CTM/29930/2017 | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/810850/EU | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/772817/EU | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/789119/EU | por |
dc.rights | openAccess | por |
dc.subject | magnetic scaffolds | por |
dc.subject | Magnetic stimulation | por |
dc.subject | Tenogenic differentiation | por |
dc.subject | TGF-β/Smad2/3 signaling pathway | por |
dc.subject | TGF-beta/Smad2/3 signaling pathway | por |
dc.title | Remote triggering of TGF-β/Smad2/3 signaling in human adipose stem cells laden on magnetic scaffolds synergistically promotes tenogenic commitment | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://doi.org/10.1016/j.actbio.2020.07.009 | por |
dc.comments | http://3bs.uminho.pt/node/20348 | por |
oaire.citationStartPage | 488 | por |
oaire.citationEndPage | 500 | por |
oaire.citationVolume | 113 | por |
dc.date.updated | 2020-08-06T11:46:10Z | - |
dc.identifier.doi | 10.1016/j.actbio.2020.07.009 | por |
dc.identifier.pmid | 32652226 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Acta Biomaterialia | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20348-MatosAM_ActaBio2020.pdf | 4,73 MB | Adobe PDF | Ver/Abrir |