Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/66347
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Amorim, Sara | por |
dc.contributor.author | Pashkuleva, I. | por |
dc.contributor.author | Reis, Celso A. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Pires, R. A. | por |
dc.date.accessioned | 2020-08-06T14:42:42Z | - |
dc.date.available | 2020-08-06T14:42:42Z | - |
dc.date.issued | 2020-03 | - |
dc.date.submitted | 2020-03 | - |
dc.identifier.citation | Amorim S., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44, Journal Of Materials Chemistry B, Vol. 17, doi:10.1039/d0tb00407c, 2020 | por |
dc.identifier.issn | 2050-750X | por |
dc.identifier.uri | https://hdl.handle.net/1822/66347 | - |
dc.description.abstract | We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion. | por |
dc.description.sponsorship | The authors acknowledge the financial support from the European Commission’s H2020 programme, under grant agreements H2020- WIDESPREAD-2014-668983-FORECAST and H2020-WIDESPREAD01-2016-2017-739572-THE DISCOVERIES CTR. S. A. acknowledges the Portuguese Foundation for Science and Technology (FCT) for her PhD grant (SFRH/BD/112075/2015) | por |
dc.language.iso | eng | por |
dc.publisher | Royal Society of Chemistry | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/668983/EU | - |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/739572/EU | - |
dc.relation | SFRH/BD/112075/2015 | - |
dc.rights | openAccess | por |
dc.subject | Bioactivity | por |
dc.subject | CD44 | por |
dc.subject | Hyaluronic acid | por |
dc.title | Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44 | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb00407c/unauth#!divAbstract | por |
dc.comments | http://3bs.uminho.pt/node/20275 | por |
oaire.citationStartPage | 3880 | por |
oaire.citationEndPage | 3885 | por |
oaire.citationIssue | 17 | por |
oaire.citationVolume | 17 | por |
dc.date.updated | 2020-08-06T14:00:40Z | - |
dc.identifier.eissn | 2050-7518 | por |
dc.identifier.doi | 10.1039/d0tb00407c | por |
dc.identifier.pmid | 32222753 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Materials Chemistry B | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20275-d0tb00407c.pdf | 2,58 MB | Adobe PDF | Ver/Abrir |