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https://hdl.handle.net/1822/65214
Título: | In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome |
Autor(es): | Cambeiro-Pérez, Noelia Hidalgo-Cantabrana, Claudio Moro-García, Marco Antonio Blanco-Míguez, Aitor Fdez-Riverola, Florentino Riestra, Sabino Lourenço, Anália Alonso-Arias, Rebeca Margolles, Abelardo Martínez-Carballo, Elena Sánchez, Borja |
Palavras-chave: | Extracellular proteins Tolerance Anti-inflammatory Treg Innate immune system |
Data: | Jul-2020 |
Editora: | Elsevier 1 |
Revista: | Journal of Functional Foods |
Citação: | Cambeiro-Pérez, Noelia; Hidalgo-Cantabrana, Claudio; Moro-García, Marco Antonio; Blanco-Míguez, Aitor; Fdez-Riverola, Florentino; Riestra, Sabino; Lourenço, Anália; Alonso-Arias, Rebeca; Margolles, Abelardo; Martínez-Carballo, Elena; Sánchez, Borja, In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome. Journal of Functional Foods, 70(103969), 2020 |
Resumo(s): | This work supports the massive presence of potential immunomodulatory peptides in the human gut metaproteome. These peptides were identified through the MAHMI database as potentially anti-inflammatory, and sixteen of them synthesized for characterize their mechanism of action. From them, peptide HM14 was encrypted in an extracellular protein produced by Bifidobacterium longum, a common member of the human microbiota, and displayed the highest anti-inflammatory capability. Molecular mechanism of action of HM14 pointed to a specific interaction between this immunomodulatory peptide and antigen presenting cells, which resulted in a higher formation of iTreg cells. Moreover, HM14 was effective in decreasing pro-inflammatory parameters in PBMCs isolated from a cohort of Crohns patients. Finally, non-targeted metabolomics confirmed the ability of HM14 to modulate the metabolic activity of PBMCs to fulfil its energy and biosynthetic requirements. Overall, our combined in silico/multiomics approach supports the human gut metaproteome as a source for immunomodulatory peptides. |
Tipo: | Artigo |
Descrição: | Supplementary data to this article can be found online at https:// doi.org/10.1016/j.jff.2020.103969. |
URI: | https://hdl.handle.net/1822/65214 |
DOI: | 10.1016/j.jff.2020.103969 |
ISSN: | 17564646 |
Versão da editora: | https://www.journals.elsevier.com/journal-of-functional-foods |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53658_1.pdf | 1,41 MB | Adobe PDF | Ver/Abrir |