Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64912
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Chaves, Susana Patrícia | por |
dc.contributor.author | Dias, Nicolina Marques | por |
dc.contributor.author | Melo, Luís Daniel Rodrigues | por |
dc.contributor.author | Azeredo, Joana | por |
dc.contributor.author | Santos, Sílvio Roberto Branco | por |
dc.contributor.author | Carvalho, Carla Alexandra Oliveira Cunha Medeiros | por |
dc.date.accessioned | 2020-04-17T09:13:29Z | - |
dc.date.available | 2020-04-17T09:13:29Z | - |
dc.date.issued | 2020-04-10 | - |
dc.identifier.citation | Costa, Susana P.; Dias, Nicolina M.; Melo, Luís D. R.; Azeredo, Joana; Santos, Sílvio B.; Carvalho, Carla M., A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood. Scientific Reports, 10(6260), 2020 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/64912 | - |
dc.description.abstract | Bloodstream infections (BSIs) are considered a major cause of death worldwide. Staphylococcus spp. are one of the most BSIs prevalent bacteria, classified as high priority due to the increasing multidrug resistant strains. Thus, a fast, specific and sensitive method for detection of these pathogens is of extreme importance. In this study, we have designed a novel assay for detection of Staphylococcus in blood culture samples, which combines the advantages of a phage endolysin cell wall binding domain (CBD) as a specific probe with the accuracy and high-throughput of flow cytometry techniques. In order to select the biorecognition molecule, three different truncations of the C-terminus of Staphylococcus phage endolysin E-LM12, namely the amidase (AMI), SH3 and amidase+SH3 (AMI_SH3) were cloned fused with a green fluorescent protein. From these, a higher binding efficiency to Staphylococcus cells was observed for AMI_SH3, indicating that the amidase domain possibly contributes to a more efficient binding of the SH3 domain. The novel phage endolysin-based flow cytometry assay provided highly reliable and specific detection of 15 CFU of Staphylococcus in 10mL of spiked blood, after 16hours of enrichment culture. Overall, the method developed herein presents advantages over the standard BSIs diagnostic methods, potentially contributing to an early and effective treatment of BSIs. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project Phages-on-chip PTDC/BTM-SAL/32442/2017 (POCI-01-0145-FEDER-032442) and the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Susana Costa was supported by the grant SFRH/BD/130098/2017 from FCT. We would also like to acknowledge Professor Hermínia de Lencastre, Doctor Carina Almeida, and Doctor Nuno Cerca for gently providing some of the strains used in this study. We acknowledge Professor Paulo Freitas for providing some ohf the infrastructures to perform the experiments. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Publishing Group | por |
dc.relation | PTDC/BTM-SAL/32442/2017 | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.relation | SFRH/BD/130098/2017 | por |
dc.rights | openAccess | por |
dc.title | A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.nature.com/srep/index.html | por |
dc.comments | CEB53637 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 10 | por |
dc.date.updated | 2020-04-11T10:25:24Z | - |
dc.identifier.eissn | 2045-2322 | por |
dc.identifier.doi | 10.1038/s41598-020-62533-7 | por |
dc.identifier.pmid | 32277078 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53637_1.pdf | 2,17 MB | Adobe PDF | Ver/Abrir |