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https://hdl.handle.net/1822/64838
Título: | Peptide nucleic acid probe, kit and method for detection and/or quantification of Escherichia coli O157:H7 and applications thereof |
Autor(es): | Almeida, Carina Sousa, J. Rocha, Rui Cerqueira, Laura Vieira, M. J. Azevedo, N. |
Data: | 6-Nov-2014 |
Citação: | Almeida, Carina; Sousa, J.; Rocha, Rui; Cerqueira, L.; Vieira, Maria João; Azevedo, N., Peptide nucleic acid probe, kit and method for detection and/or quantification of Escherichia coli O157:H7 and applications thereof. International Patent, WO 2014/178741 A2, 2014. |
Resumo(s): | PNA is a synthetic molecule analogue to DNA that, due to its physicochemical properties, allows a faster analysis with higher sensitivity and specificity than the DNA probes. These probes are combined with fluorescence in situ hybridization (FISH), a molecular biology technique that allows the direct visualization of the microorganisms in the sample. The combination of these two technologies rendered the FISH procedure to be faster, simpler and more efficient. This method can be applied to a great variety of samples such as food, blood, biopsies, feces, water and other clinical, environmental or agriculture and food industry samples. The present invention also includes the development of the kit of detection and respective procedure for the specific identification of Escherichia coli O157:H7 using the above referred sample types. |
Tipo: | Patente |
Descrição: | International Patent: US/2016/0273025 (22-09-2016) |
URI: | https://hdl.handle.net/1822/64838 |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_15030_1.pdf | 1,44 MB | Adobe PDF | Ver/Abrir |