Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64832
Título: | Assessing autophagy in archived tissue or how to capture autophagic flux from a tissue snapshot |
Autor(es): | Humbert, Magali Morán, María de la Cruz-Ojeda, Patricia Muntané, Jordi Wiedmer, Tabea Apostolova, Nadezda McKenna, Sharon L. Velasco, Guillermo Balduini, Walter Eckhart, Leopold Janji, Bassam Marques, Maria Belém Sousa Sampaio Ludovico, Paula Žerovnik, Eva Langer, Rupert Perren, Aurel Engedal, Nikolai Tschan, Mario P. |
Palavras-chave: | autophagy biomarkers pathology disease |
Data: | 21-Mar-2020 |
Editora: | Multidisciplinary Digital Publishing Institute |
Revista: | Biology |
Citação: | Humbert, M.; Morán, M.; de la Cruz-Ojeda, P.; Muntané, J.; Wiedmer, T.; Apostolova, N.; McKenna, S.L.; Velasco, G.; Balduini, W.; Eckhart, L.; Janji, B.; Sampaio-Marques, B.; Ludovico, P.; Žerovnik, E.; Langer, R.; Perren, A.; Engedal, N.; Tschan, M.P. Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot. Biology 2020, 9, 59. |
Resumo(s): | Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64832 |
DOI: | 10.3390/biology9030059 |
e-ISSN: | 2079-7737 |
Versão da editora: | https://www.mdpi.com/2079-7737/9/3/59 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
biology-09-00059.pdf | 1,29 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons