Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64331
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Campo DC | Valor | Idioma |
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dc.contributor.author | Kallscheuer, Nicolai | por |
dc.contributor.author | Menezes, Regina | por |
dc.contributor.author | Foito, Alexandre | por |
dc.contributor.author | Henriques da Silva, Marcelo D. | por |
dc.contributor.author | Braga, Adelaide | por |
dc.contributor.author | Dekker, Wijbrand | por |
dc.contributor.author | Sevillano, David Méndez | por |
dc.contributor.author | Rosado-Ramos, Rita | por |
dc.contributor.author | Jardim, Carolina | por |
dc.contributor.author | Oliveira, Joana Filipa Azevedo | por |
dc.contributor.author | Ferreira, Patrícia | por |
dc.contributor.author | Rocha, I. | por |
dc.contributor.author | Silva, Ana Rita | por |
dc.contributor.author | Sousa, M. | por |
dc.contributor.author | Allwood, James William | por |
dc.contributor.author | Bott, Michael | por |
dc.contributor.author | Faria, Nuno Costa Martins de | por |
dc.contributor.author | Stewart, Derek | por |
dc.contributor.author | Ottens, Marcel | por |
dc.contributor.author | Naesby, Michael | por |
dc.contributor.author | Santos, Claudia Nunes dos | por |
dc.contributor.author | Marienhagen, Jan | por |
dc.date.accessioned | 2020-03-10T09:11:19Z | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | Kallscheuer, Nicolai; Menezes, Regina; Foito, Alexandre; Henriques da Silva, Marcelo D.; Braga, Adelaide; Dekker, Wijbrand; Sevillano, David Méndez; Rosado-Ramos, Rita; Jardim, Carolina; Oliveira, J.; Ferreira, Patrícia; Rocha, Isabel; Silva, Ana Rita; Sousa, M.; Allwood, James William; Bott, Michael; Faria, N.; Stewart, Derek; Ottens, Marcel; Naesby, Michael; Santos, Claudia Nunes dos; Marienhagen, Jan, Identification and microbial production of the raspberry phenol salidroside that is active against Huntington's disease. Plant Physiology, 179(3), 969-985, 2019 | por |
dc.identifier.issn | 0032-0889 | por |
dc.identifier.uri | https://hdl.handle.net/1822/64331 | - |
dc.description | Sequence data from this article can be found in the GenBank/EMBL data libraries under accession numbers: KX262844.1 (RsUGT72B14, R. sachalinensis); AY547304.1 (RsUGT73B6. R. sachalinensis); NP_418458.1 (malE, E. coli); NP_415214.1 (pgm, E. coli); NP_415752.1 (galU, E. coli); NM_001180688.3 (aro10, S. cerevisiae), and NP_417484.1 (yqhD, E. coli). | por |
dc.description.abstract | Edible berries are considered to be among nature's treasure chests as they contain a large number of (poly)phenols with potentially health-promoting properties. However, as berries contain complex (poly)phenol mixtures, it is challenging to associate any interesting pharmacological activity with a single compound. Thus, identification of pharmacologically interesting phenols requires systematic analyses of berry extracts. Here, raspberry (Rubus idaeus, var. Prestige) extracts were systematically analyzed to identify bioactive compounds against pathological processes of neurodegenerative diseases. Berry extracts were tested on different Saccharomyces cerevisiae strains expressing disease proteins associated with Alzheimer's, Parkinson's, or Huntington's disease or amyotrophic lateral sclerosis. After identifying bioactivity against Huntington's disease, the extract was fractionated and the obtained fractions were tested in the yeast model, which revealed that salidroside, a glycosylated phenol, displayed significant bioactivity. Subsequently, a metabolic route to salidroside was reconstructed in S. cerevisiae and Corynebacterium glutamicum. The best-performing S. cerevisiae strain was capable of producing 2.1 mM (640 mg L-1) salidroside from glucose in shake flasks, whereas an engineered C. glutamicum strain could efficiently convert the precursor tyrosol to salidroside, accumulating up to 32 mM (9,700 mg L-1) salidroside in bioreactor cultivations (yield: 0.81 mol mol-1). Targeted yeast assays verified that salidroside produced by both organisms has the same positive effects as salidroside of natural origin. | por |
dc.description.sponsorship | We express our gratitude to Dr. Rute Neves (Chr. Hansen A/S, Denmark), Prof. Dr. Jochen Förster and Dr. Alexey Dudnik (The Nova Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark), who coordinated the BacHBerry project. We also thank Prof. Dr. Ian Macreadie (Centre of Excellence for Alzheimer’s Disease Research & Care, School of Exercise, Biomedical & Health Sciences, Edith Cowan University, WA, Australia) for providing p416_GPD-GFP_AB42; Prof. Dr. Tiago Outeiro (University Medical Center Gottingen, Department of Neurodegeneration and Restorative Research, Germany) for providing W303-1A_Syn and W303-1A TU; Prof. Dr. Flaviano Giorgini (Department of Genetics and Genome Biology, University of Leicester, UK) for providing p425GAL1_HTT103Q; and Prof. Dr. Greg Petsko (Department of Biochemistry and Chemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, U.S.) for providing pYES_GAL1pr-FUS-GFP and pYES_CT. | por |
dc.language.iso | eng | por |
dc.publisher | American Society of Plant Biologists | por |
dc.rights | restrictedAccess | por |
dc.title | Identification and microbial production of the raspberry phenol salidroside that is active against Huntington's disease | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.plantphysiol.org/ | por |
dc.comments | CEB49120 | por |
oaire.citationStartPage | 969 | por |
oaire.citationEndPage | 985 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 179 | por |
dc.date.updated | 2020-03-07T13:36:55Z | - |
dc.identifier.eissn | 1532-2548 | por |
dc.identifier.doi | 10.1104/pp.18.01074 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 30397021 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Plant Physiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_49120_1.pdf Acesso restrito! | 2,42 MB | Adobe PDF | Ver/Abrir |