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https://hdl.handle.net/1822/64318
Título: | Comparison of antiproliferative effect of epigallocatechin gallate when loaded into cationic solid lipid nanoparticles against different cell lines |
Autor(es): | Silva, Amélia M. Martins-Gomes, Carlos Fangueiro, Joana F. Andreani, Tatiana Souto, Eliana B. |
Palavras-chave: | Solid Lipid nanoparticles Nanoencapsulation Epigalloacatechin-gallate Anti-proliferative effect Cytotoxicity Cationic Lipids |
Data: | Out-2019 |
Editora: | Marcel Dekker Inc. |
Revista: | Pharmaceutical Development and Technology |
Citação: | Silva, Amélia M.; Martins-Gomes, Carlos; Fangueiro, Joana F.; Andreani, Tatiana; Souto, Eliana, Comparison of antiproliferative effect of epigallocatechin gallate when loaded into cationic solid lipid nanoparticles against different cell lines. Pharmaceutical Development and Technology, 24(10), 1243-1249, 2019 |
Resumo(s): | Several therapeutic properties have been attributed to epigallocatechin gallate (EGCG), a phytopharmaceutical polyphenol with antioxidant and antiproliferative activity. EGCG is however very prone to oxidation in aqueous solutions which changes its bioactive properties. Its loading in nanoparticles has been proposed to reduce its degradation while increasing its in vivo efficacy. The aim of this study was to compare the antiproliferative effect of EGCG before and after its loading in solid lipid nanoparticles (SLNs), against five different cell lines (Caco-2, HepG2, MCF-7, SV-80 and Y-79). EGCG produced concentration- and time-dependent antiproliferative effect, with eficacy dependent on the cell line. The order of potency was: MCF-7?>?SV-80?>?HepG2?>?Y-79?>?Caco-2, for 24h exposure (MCF-7 IC50=58.60?±?3.29 µg/mL; Caco-2 IC50>500.00 µg/mL). To the best of our knowledge this is the first study reporting EGCG antiproliferative effect in SV-80 and Y-79 cells. DDAB-SLN physicochemical properties (size ?134nm; PI?0.179; ZP ?+28mV) were only slightly modified with EGCG loading (EGCG-DDAB-SLN: ?144nm; PI?0.160; ZP ?+26mV). EGCG loadingin SLN, only slightly increases the EGCG antiproliferative effect in MCF-7 and SV-80 cells. SLN exhibited intrinsic toxicity, attributed to the surfactant used in its production. From the obtained results, the biocompatibility of blank SLN must be also considered when testing the efficacy of loaded phytopharmaceutics. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64318 |
DOI: | 10.1080/10837450.2019.1658774 |
ISSN: | 1083-7450 |
e-ISSN: | 1097-9867 |
Versão da editora: | https://www.tandfonline.com/loi/iphd20 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51961_1.pdf | 808,96 kB | Adobe PDF | Ver/Abrir |