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https://hdl.handle.net/1822/64309
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Campo DC | Valor | Idioma |
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dc.contributor.author | Vasconcelos, Natália L. | por |
dc.contributor.author | Gomes, Eduardo Domingos Correia | por |
dc.contributor.author | Oliveira, Maria Eduarda Moreira Pinheiro Santos | por |
dc.contributor.author | Silva, Carlos J. R. | por |
dc.contributor.author | Lima, Rui | por |
dc.contributor.author | Sousa, Nuno | por |
dc.contributor.author | Salgado, A. J. | por |
dc.contributor.author | Silva, Nuno André Martins | por |
dc.date.accessioned | 2020-03-06T15:36:30Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1529-9430 | - |
dc.identifier.uri | https://hdl.handle.net/1822/64309 | - |
dc.description.abstract | BACKGROUND CONTEXT: Damage to the spinal cord can result in irreversible impairments or complete loss of motor, sensory, and autonomic functions. Riluzole and magnesium have been widely investigated as neuroprotective agents in animal models of spinal cord injury. As these drugs protect the injured spinal cord through different mechanisms, we aimed to investigate if their neuroprotective efficacy could be cumulative. PURPOSE: This study aimed to investigate the neuroprotective efficacy of combined administration of riluzole and magnesium chloride in a contusive model of thoracic spinal cord injury. STUDY DESIGN: An in vivo experiment was set using female Wistar Han rats that underwent a thoracic spinal cord contusion (T8) using a weight drop method. An hour after injury, animals were randomly distributed to receive (1) saline, (2) riluzole (2.50 mg/kg), (3) magnesium chloride (24.18 mg/kg) in a polyethylene glycol formulation, or (4) a combined treatment (riluzole and magnesium). Subsequent treatments were given in four intraperitoneal injections (spaced 12 hours apart). METHODS: The Basso, Beattie, and Bresnahan locomotor rating scale, an activity box test, and a swimming test were used to evaluate behavioral recovery over a 4-week period. Histologic analysis of the spinal cords was performed to measure the extent and volume of the lesion, axonal preservation, serotonergic and glutamatergic fiber sparing, motor neuron survival, and inflammation. RESULTS: Our results show that only the riluzole treatment significantly improved behavioral recovery up to 4 weeks after injury when compared with saline controls (6.2±1.8), with animals achieving weight-supported stepping (9.1±1.2). Riluzole also promoted tissue sparing with significant differences achieved from 200 to 600 m (caudally to the lesion epicenter), and reduced lesion volume, with animals presenting a significantly smaller lesion (3.23±0.26 mm3 ) when compared with the saline-treated group (4.74±0.80 mm3 ), representing a 32% decrease in lesion volume. Riluzole treatment induced significant axonal preservation, as well as serotonergic fiber sparing, caudally to the injury epicenter. CONCLUSIONS: Our results suggest that the combined treatment, although simultaneously targeting two excitotoxic-related mechanisms, did not further improve behavioral and histologic outcome when compared with riluzole given alone. | eng |
dc.description.sponsorship | Prémios Santa Casa Neurociências – Prize Melo e Castro for Spinal Cord Injury Research; Portuguese Foundation for Science and Technology (Post-Doctoral fellowship – SFRH/ BPD/97701/2013 – to NA Silva; IF Development Grant to AJ Salgado); This article is a result of the project (NORTE01-0145-FEDER-000013), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); Cofinanciado pelo Programa Operacional Regional do Norte (ON.2 SR&TD Integrated Program – NORTE-07-0124-FEDER-000021), ao abrigo do Quadro de Referência Estratégico Nacional (QREN), através do Fundo Europeu de Desenvolvimento Regional (FEDER); Projeto Estratégico – LA 26 – 2011–2012 and Projeto Estratégico – LA 26 – 2013–2014 cofinanciado por fundos nacionais, através da Fundação para a Ciência e a Tecnologia (PEst-C/SAU/LA0026/2011; PEst-C/SAU/ LA0026/2013), e pelo Fundo Europeu de Desenvolvimento Regional (FEDER), através do COMPETE (FCOMP-01- 0124-FEDER-022724; FCOMP-01-0124-FEDER-037298). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F97701%2F2013/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/133016/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Animals | por |
dc.subject | Drug therapy, combination | por |
dc.subject | Female | por |
dc.subject | Magnesium | por |
dc.subject | Neuroprotective agents | por |
dc.subject | Rats | por |
dc.subject | Rats, wistar | por |
dc.subject | Riluzole | por |
dc.subject | Spinal cord Injuries | por |
dc.subject | Excitotoxicity | por |
dc.subject | Ionic imbalances | por |
dc.subject | Neuroprotection | por |
dc.subject | Spinal cord injury | por |
dc.title | Combining neuroprotective agents: effect of riluzole and magnesium in a rat model of thoracic spinal cord injury | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.thespinejournalonline.com/article/S1529-9430(16)30041-9/abstract | por |
oaire.citationStartPage | 1015 | por |
oaire.citationEndPage | 1024 | por |
oaire.citationIssue | 8 | por |
oaire.citationVolume | 16 | por |
dc.identifier.doi | 10.1016/j.spinee.2016.04.013 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.eisbn | 1878-1632 | - |
dc.identifier.pmid | 27109831 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | The Spine Journal | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
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Ficheiro | Descrição | Tamanho | Formato | |
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Vasconcelos-2016-Combining-neuroprotective-agents-ef.pdf Acesso restrito! | 2,37 MB | Adobe PDF | Ver/Abrir |