Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64214
Título: | Silver nanoparticles-composing alginate/gelatine hydrogel improves wound healing in vivo |
Autor(es): | Diniz, Flavia Resende Maia, Romerito Cesar A. P. Andrade, Lucas Rannier Andrade, Luciana Nalone Vinicius Chaud, Marco Silva, Classius Ferreira da Corrêa, Cristiane Bani Junior, Ricardo Luiz C. de Albuquerque Costa, Luiz Pereira da Shin, Su Ryon Hassan, Shabir Sanchez-Lopez, Elena Souto, Eliana B. Severino, Patricia |
Palavras-chave: | sodium alginate gelatin silver nanoparticles antimicrobial activity healing |
Data: | 2020 |
Editora: | MDPI |
Revista: | Nanomaterials |
Citação: | Diniz, Flavia Resende; Maia, Romerito Cesar A. P.; Rannier Andrade, Lucas; Andrade, Luciana Nalone; Vinicius Chaud, Marco; da Silva, Classius Ferreira; Corrêa, Cristiane Bani; de Albuquerque Junior, Ricardo Luiz C.; Pereira da Costa, Luiz; Shin, Su Ryon; Hassan, Shabir; Sanchez-Lopez, Elena; Souto, Eliana; Severino, Patricia, Silver nanoparticles-composing alginate/gelatine hydrogel improves wound healing in vivo. Nanomaterials, 10(2), 390, 2020 |
Resumo(s): | Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO3 precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430450 nm in the ultraviolet-visible (UVVis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FTIR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm1. Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against Pseudomonas aeruginosa and 53.0 µg/mL against Staphylococcus aureus. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64214 |
DOI: | 10.3390/nano10020390 |
ISSN: | 2079-4991 |
Versão da editora: | http://www.mdpi.com/journal/nanomaterials |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53537_1.pdf | 7,76 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons