Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64154
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Souto, Eliana B. | por |
dc.contributor.author | Souto, Selma B. | por |
dc.contributor.author | Zielinska, Aleksandra | por |
dc.contributor.author | Durazzo, Alessandra | por |
dc.contributor.author | Lucarini, Massimo | por |
dc.contributor.author | Santini, Antonello | por |
dc.contributor.author | Horbánkczuk, Olaf K. | por |
dc.contributor.author | Atanasov, Atanas G. | por |
dc.contributor.author | Marques, Conrado | por |
dc.contributor.author | Andrade, Luciana N. | por |
dc.contributor.author | Silva, Amélia M. | por |
dc.contributor.author | Severino, Patricia | por |
dc.date.accessioned | 2020-03-02T15:27:45Z | - |
dc.date.available | 2020-03-02T15:27:45Z | - |
dc.date.issued | 2020-02-16 | - |
dc.identifier.citation | Souto, Eliana; Souto, Selma B.; Zielinska, Aleksandra; Durazzo, Alessandra; Lucarini, Massimo; Santini, Antonello; Horbánkczuk, Olaf K.; Atanasov, Atanas G.; Marques, Conrado; Andrade, Luciana N.; Silva, Amélia M.; Severino, Patricia, Perillaldehyde 1,2-epoxide loaded SLN-tailored mAb: production, physicochemical characterization and in vitro cytotoxicity profile in MCF-7 cell lines. Pharmaceutics, 12(2), 161, 2020. | por |
dc.identifier.issn | 1999-4923 | por |
dc.identifier.uri | https://hdl.handle.net/1822/64154 | - |
dc.description.abstract | We have developed a new cationic solid lipid nanoparticle (SLN) formulation, composed of Compritol ATO 888, poloxamer 188 and cetyltrimethylammonium bromide (CTAB), to load perillaldehyde 1,2-epoxide, and surface-tailored with a monoclonal antibody for site-specific targeting of human epithelial growth receptor 2 (HER2). Perillaldehyde 1,2-epoxide-loaded cationic SLN (cPa-SLN), with a mean particle size (z-Ave) of 275.31 ± 4.78 nm and polydispersity index (PI) of 0.303 ± 0.081, were produced by high shear homogenization. An encapsulation efficiency of cPa-SLN above 80% was achieved. The release of perillaldehyde 1,2-epoxide from cationic SLN followed the Korsemeyer–Peppas kinetic model, which is typically seen in nanoparticle formulations. The lipid peroxidation of cPa-SLN was assessed by the capacity to produce thiobarbituric acid-reactive substances, while the antioxidant activity was determined by the capacity to scavenge the stable radical DPPH. The surface functionalization of cPa-SLN with the antibody was done via streptavidin-biotin interaction, monitoring z-Ave, PI and ZP of the obtained assembly (cPa-SLN-SAb), as well as its stability in phosphate buffer. The effect of plain cationic SLN (c-SLN, monoterpene free), cPa-SLN and cPa-SLN-SAb onto the MCF-7 cell lines was evaluated in a concentration range from 0.01 to 0.1 mg/mL, confirming that streptavidin adsorption onto cPa-SLN-SAb improved the cell viability in comparison to the cationic cPa-SLN. | por |
dc.description.sponsorship | The authors wish to acknowledge the financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), and from FAPITEC/SE (Fundação de Apoio à Pesquisa e Inovação Tecnológica do Estado de Sergipe). E.B. Souto acknowledges the sponsorship of the projects M-ERA-NET-0004/2015-PAIRED and UIDB/04469/2020, receiving support from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, and co-financed by FEDER, under the Partnership Agreement PT2020. | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.relation | UIDB/04469/2020 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | perillaldehyde 1,2-epoxide | por |
dc.subject | Compritol ATO 888 | por |
dc.subject | cationic SLN | por |
dc.subject | streptavidin adsorption | por |
dc.subject | MCF-7 cells | por |
dc.subject | perillaldehyde 1 | por |
dc.subject | 2-epoxide | por |
dc.title | Perillaldehyde 1,2-epoxide loaded SLN-tailored mAb: production, physicochemical characterization and in vitro cytotoxicity profile in MCF-7 cell lines | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/journal/pharmaceutics | por |
dc.comments | CEB53516 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 12 | por |
dc.date.updated | 2020-02-22T20:43:12Z | - |
dc.identifier.eissn | 1999-4923 | por |
dc.identifier.doi | 10.3390/pharmaceutics12020161 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Pharmaceutics | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53516_1.pdf | 1,28 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons