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https://hdl.handle.net/1822/63305
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Campo DC | Valor | Idioma |
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dc.contributor.author | Faria-Oliveira, Fábio | por |
dc.contributor.author | Carvalho, Joana | por |
dc.contributor.author | Belmiro, Celso L R | por |
dc.contributor.author | Martinez-Gomariz, Montserrat | por |
dc.contributor.author | Hernaez, Maria Luisa | por |
dc.contributor.author | Pavão, Mauro | por |
dc.contributor.author | Gil, Concha | por |
dc.contributor.author | Lucas, Cândida | por |
dc.contributor.author | Ferreira, Célia | por |
dc.date.accessioned | 2020-01-20T12:33:16Z | - |
dc.date.available | 2020-01-20T12:33:16Z | - |
dc.date.issued | 2014-10-25 | - |
dc.identifier.issn | 1471-2180 | por |
dc.identifier.uri | https://hdl.handle.net/1822/63305 | - |
dc.description.abstract | In a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support and protect bacterial and fungal multicellular aggregates, such as biofilms or colonies. However, the roles and composition of this microbial ECM are still poorly understood. | por |
dc.description.sponsorship | Authors would like to acknowledge Joana Tulha for assistance on yeasts overlay photographs, and to Rui Armada for C. albicans ECM SDS-PAGE experiment. The proteomic analysis was carried out at the proteomics facility UCM-PCM, a member of the ProteoRed network. The polysaccharide analysis was performed at the Laboratory of Glycoconjugates Biochemistry and Cellular Biology, UFRJ, Brazil. Fabio Faria-Oliveira was supported by a PhD scholarship SFRH/BD/45368/2008 from FCT (Fundacao para a Ciencia e a Tecnologia). This work was funded by Marie Curie Initial Training Network GLYCOPHARM (PITN-GA-2012-317297), and by FCT/MEC through Portuguese funds (PIDDAC) - PEst-OE/BIA/UI4050/2014. The authors would also like to acknowledge Hugh S. Johnson for critical reading of the manuscript regarding English usage. | por |
dc.language.iso | eng | por |
dc.publisher | BMC | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F45368%2F2008/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/135919/PT | por |
dc.rights | openAccess | por |
dc.subject | Candida albicans | por |
dc.subject | Chemistry Techniques, Analytical | por |
dc.subject | Extracellular Matrix | por |
dc.subject | Fungal Proteins | por |
dc.subject | Glycomics | por |
dc.subject | Polysaccharides | por |
dc.subject | Proteomics | por |
dc.subject | Reproducibility of Results | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.title | Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 14 | por |
dc.identifier.doi | 10.1186/s12866-014-0244-0 | por |
dc.identifier.pmid | 25344425 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | BMC Microbiology | por |
oaire.version | VoR | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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s12866-014-0244-0.pdf | 975,34 kB | Adobe PDF | Ver/Abrir |