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https://hdl.handle.net/1822/62008
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Campo DC | Valor | Idioma |
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dc.contributor.author | Batista, Andreia F. R. | por |
dc.contributor.author | Martínez, José C. | por |
dc.contributor.author | Hengst, Ulrich | por |
dc.date.accessioned | 2019-11-11T11:08:48Z | - |
dc.date.issued | 2017-09-26 | - |
dc.identifier.citation | Batista, A. F., Martínez, J. C., & Hengst, U. (2017). Intra-axonal synthesis of SNAP25 is required for the formation of presynaptic terminals. Cell reports, 20(13), 3085-3098 | por |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | https://hdl.handle.net/1822/62008 | - |
dc.description.abstract | Localized protein synthesis is a mechanism for developing axons to react acutely and in a spatially restricted manner to extracellular signals. As such, it is important for many aspects of axonal development, but its role in the formation of presynapses remains poorly understood. We found that the induced assembly of presynaptic terminals required local protein synthesis. Newly synthesized proteins were detectable at nascent presynapses within 15 min of inducing synapse formation in isolated axons. The transcript for the t-SNARE protein SNAP25, which is required for the fusion of synaptic vesicles with the plasma membrane, was recruited to presynaptic sites and locally translated. Inhibition of intra-axonal SNAP25 synthesis affected the clustering of SNAP25 and other presynaptic proteins and interfered with the release of synaptic vesicles from presynaptic sites. This study reveals a critical role for the axonal synthesis of SNAP25 in the assembly of presynaptic terminals. | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT) in the context of the FCT funded University of Minho MD/PhD Program (SFRH/BD/52322/2013). J.C.M. was supported by the National Institutes of Health (F31GM116617). U.H. was supported by the NIH (R01MH096702), the BrightFocus Foundation (A2015093S), and the Irma T. Hirschl Trust | por |
dc.language.iso | eng | por |
dc.publisher | Cell Press | por |
dc.rights | closedAccess | por |
dc.subject | Animals | por |
dc.subject | Axons | por |
dc.subject | Embryo, Mammalian | por |
dc.subject | Presynaptic Terminals | por |
dc.subject | Rats | por |
dc.subject | Rats, Sprague-Dawley | por |
dc.subject | Synaptosomal-Associated Protein 25 | por |
dc.subject | axon | por |
dc.subject | local protein synthesis | por |
dc.subject | presynapse | por |
dc.subject | SNAP25 | por |
dc.subject | synapse formation | por |
dc.subject | synaptic vesicle release | por |
dc.subject | β-catenin | por |
dc.title | Intra-axonal Synthesis of SNAP25 Is Required for the Formation of Presynaptic Terminals | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S2211124717312573 | por |
oaire.citationStartPage | 3085 | por |
oaire.citationEndPage | 3098 | por |
oaire.citationIssue | 13 | por |
oaire.citationVolume | 20 | por |
dc.identifier.eissn | 2211-1247 | - |
dc.identifier.doi | 10.1016/j.celrep.2017.08.097 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 28954226 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Cell Reports | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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batista2017.pdf Acesso restrito! | 7,33 MB | Adobe PDF | Ver/Abrir |