Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61999
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Rothchild, Alissa C. | por |
dc.contributor.author | Jayaraman, Pushpa | por |
dc.contributor.author | Alves, Cláudio | por |
dc.contributor.author | Behar, Samuel M. | por |
dc.date.accessioned | 2019-11-08T16:39:47Z | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | Rothchild, A. C., Jayaraman, P., Nunes-Alves, C., & Behar, S. M. (2014). iNKT cell production of GM-CSF controls Mycobacterium tuberculosis. PLoS pathogens, 10(1), e1003805. | por |
dc.identifier.issn | 1553-7366 | - |
dc.identifier.uri | https://hdl.handle.net/1822/61999 | - |
dc.description.abstract | Invariant natural killer T (iNKT) cells are activated during infection, but how they limit microbial growth is unknown in most cases. We investigated how iNKT cells suppress intracellular Mycobacterium tuberculosis (Mtb) replication. When co-cultured with infected macrophages, iNKT cell activation, as measured by CD25 upregulation and IFNγ production, was primarily driven by IL-12 and IL-18. In contrast, iNKT cell control of Mtb growth was CD1d-dependent, and did not require IL-12, IL-18, or IFNγ. This demonstrated that conventional activation markers did not correlate with iNKT cell effector function during Mtb infection. iNKT cell control of Mtb replication was also independent of TNF and cell-mediated cytotoxicity. By dissociating cytokine-driven activation and CD1d-restricted effector function, we uncovered a novel mediator of iNKT cell antimicrobial activity: GM-CSF. iNKT cells produced GM-CSF in vitro and in vivo in a CD1d-dependent manner during Mtb infection, and GM-CSF was both necessary and sufficient to control Mtb growth. Here, we have identified GM-CSF production as a novel iNKT cell antimicrobial effector function and uncovered a potential role for GM-CSF in T cell immunity against Mtb. | por |
dc.description.sponsorship | This work was supported by National Institutes of Health (NIH) R01HL080330 to SMB, T32AR007530 supporting ACR, the American Lung Association postdoctoral research training fellowship, RT-123085-N, and Harvard University Center for AIDS Research (CFAR) Scholar Award, an NIH funded program, P30 AI060354, to PJ, and a PhD fellowship from Fundacao para a Ciencia e Tecnologia (Portugal) to CNA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | por |
dc.language.iso | eng | por |
dc.publisher | Public Library of Science (PLOS) | por |
dc.rights | restrictedAccess | por |
dc.subject | Animals | por |
dc.subject | Granulocyte-Macrophage Colony-Stimulating Factor | por |
dc.subject | Interferon-gamma | por |
dc.subject | Interleukin-12 | por |
dc.subject | Interleukin-18 | por |
dc.subject | Macrophages, Peritoneal | por |
dc.subject | Mice | por |
dc.subject | Mice, Knockout | por |
dc.subject | Mycobacterium tuberculosis | por |
dc.subject | Natural Killer T-Cells | por |
dc.subject | Tuberculosis | por |
dc.subject | Lymphocyte Activation | por |
dc.title | iNKT cell production of GM-CSF controls Mycobacterium tuberculosis | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003805 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 10 | por |
dc.identifier.eissn | 1553-7374 | - |
dc.identifier.doi | 10.1371/journal.ppat.1003805 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 24391492 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | PLoS Pathogens | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Rothchild-2014-Inkt-cell-production-of-gm-csf-cont.pdf Acesso restrito! | 986,03 kB | Adobe PDF | Ver/Abrir |