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https://hdl.handle.net/1822/61691
Título: | Antimicrobial action and clotting time of thin, hydrated poly(Vinyl Alcohol)/cellulose acetate films functionalized with LL37 for prospective wound-healing applications |
Autor(es): | Felgueiras, Helena Prado Teixeira, Marta Albertina Tavares, Tânia Daniela Eugénio Homem, Natália Cândido Zille, Andrea Amorim, M. T. Pessoa de |
Palavras-chave: | Biodegradable films Peptide LL37 Antibacterial action Accelerated clotting Wound-healing biocompatibility biodegradable bioengineering biomedical Applications films |
Data: | 2020 |
Editora: | Wiley |
Revista: | Journal of Applied Polymer Science |
Resumo(s): | Poly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈ 75% inhibition for S. aureus, ≈ 85% for S. epidermidis and ≈ 60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈ 10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/61691 |
DOI: | 10.1002/app.48626 |
ISSN: | 0021-8995 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2019.10.03_Manuscript PVA-CA black.pdf | 1,32 MB | Adobe PDF | Ver/Abrir |