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https://hdl.handle.net/1822/61485
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Campo DC | Valor | Idioma |
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dc.contributor.author | Magalhães, Andreia Patrícia Alves | por |
dc.contributor.author | França, Ângela Maria Oliveira Sousa | por |
dc.contributor.author | Pereira, Maria Olívia | por |
dc.contributor.author | Cerca, Nuno | por |
dc.date.accessioned | 2019-09-24T14:28:48Z | - |
dc.date.available | 2019-09-24T14:28:48Z | - |
dc.date.issued | 2019-09-20 | - |
dc.identifier.citation | Magalhães, Andreia P.; França, Angela; Pereira, Maria Olívia; Cerca, Nuno, RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms. Scientific Reports, 9(13639), 2019 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61485 | - |
dc.description | Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-50094-3. | por |
dc.description.abstract | While considerable research has focused on studying individual-species, we now face the challenge of determining how interspecies interactions alter bacterial behaviours and pathogenesis. Pseudomonas aeruginosa and Staphylococcus aureus are often found to co-infect cystic-fibrosis patients. Curiously, their interaction is reported as competitive under laboratory conditions. Selecting appropriate methodologies is therefore critical to analyse multi-species communities. Herein, we demonstrated the major biases associated with qPCR quantification of bacterial populations and optimized a RNA-based qPCR able not only to quantify but also to characterize microbial interactions within dual-species biofilms composed by P. aeruginosa and S. aureus, as assessed by gene expression quantification. qPCR quantification was compared with flow-cytometry and culture-based quantification. Discrepancies between culture independent and culture dependent methods could be the result of the presence of viable but not-cultivable bacteria within the biofilm. Fluorescence microscopy confirmed this. A higher sensitivity to detect viable cells further highlights the potentialities of qPCR approach to quantify biofilm communities. By using bacterial RNA and an exogenous mRNA control, it was also possible to characterize bacterial transcriptomic profile, being this a major advantage of this method. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Grant of APM (SFRH/BD/132165/2017). | por |
dc.language.iso | eng | por |
dc.publisher | Nature Research | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.relation | SFRH/BD/132165/2017 | por |
dc.rights | openAccess | por |
dc.title | RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.nature.com/srep/index.html | por |
dc.comments | CEB52020 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2019-09-21T11:14:47Z | - |
dc.identifier.eissn | 2045-2322 | por |
dc.identifier.doi | 10.1038/s41598-019-50094-3 | por |
dc.identifier.pmid | 31541147 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_52020_1.pdf | 1,62 MB | Adobe PDF | Ver/Abrir |