Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61288
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Campo DC | Valor | Idioma |
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dc.contributor.author | Akturk, Ergun | por |
dc.contributor.author | Oliveira, Hugo | por |
dc.contributor.author | Santos, Sílvio B. | por |
dc.contributor.author | Susana Patrícia Chaves | por |
dc.contributor.author | Kuyumcu, Suleyman | por |
dc.contributor.author | Melo, Luís Daniel Rodrigues | por |
dc.contributor.author | Azeredo, Joana | por |
dc.date.accessioned | 2019-09-02T15:14:01Z | - |
dc.date.available | 2019-09-02T15:14:01Z | - |
dc.date.issued | 2019-07-25 | - |
dc.identifier.citation | Akturk, Ergun; Oliveira, Hugo; Santos, Sílvio B.; Costa, Susana P.; Kuyumcu, Suleyman; Melo, Luís D. R.; Azeredo, Joana, Synergistic action of phage and antibiotics: parameters to enhance the killing efficacy against mono and dual-species biofilms. Antibiotics, 8(3), 103, 2019 | por |
dc.identifier.issn | 2079-6382 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61288 | - |
dc.description.abstract | Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens and are commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence in a biofilm contributes to an increased tolerance of the biofilm to antibiotics. Combined treatments of bacteriophages and antibiotics have shown a promising antibiofilm activity, due to the profound differences in their mechanisms of action. In this study, 48 h old mono and dual-species biofilms were treated with a newly isolated P. aeruginosa infecting phage (EPA1) and seven different antibiotics (gentamicin, kanamycin, tetracycline, chloramphenicol, erythromycin, ciprofloxacin, and meropenem), alone and in simultaneous or sequential combinations. The therapeutic efficacy of the tested antimicrobials was determined. Phage or antibiotics alone had a modest effect in reducing biofilm bacteria. However, when applied simultaneously, a profound improvement in the killing effect was observed. Moreover, an impressive biofilm reduction (below the detection limit) was observed when gentamicin or ciprofloxacin were added sequentially after 6 h of phage treatment. The effect observed does not depend on the type of antibiotic but is influenced by its concentration. Moreover, in dual-species biofilms it was necessary to increase gentamicin concentration to obtain a similar killing effect as occurs in mono-species. Overall, combining phages with antibiotics can be synergistic in reducing the bacterial density in biofilms. However, the concentration of antibiotic and the time of antibiotic application are essential factors that need to be considered in the combined treatments. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684) and the Project PTDC/BBB-BSS/6471/2014 (POCI-01-0145-FEDER-016678). This work was also supported by BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Ergun Akturk acknowledges FCT for grant PD/BD/135254/2017. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI AG | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | PTDC/BBB-BSS/6471/2014 | por |
dc.relation | PD/BD/135254/2017 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Pseudomonas aeruginosa | por |
dc.subject | Staphylococcus aureus | por |
dc.subject | bacteriophage | por |
dc.subject | dual-species | por |
dc.subject | biofilms | por |
dc.subject | antibiotic | por |
dc.subject | synergy | por |
dc.subject | simultaneous | por |
dc.subject | sequential | por |
dc.title | Synergistic action of phage and antibiotics: parameters to enhance the killing efficacy against mono and dual-species biofilms | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/journal/antibiotics | por |
dc.comments | CEB51941 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 8 | por |
dc.date.updated | 2019-08-18T14:18:36Z | - |
dc.identifier.eissn | 2079-6382 | por |
dc.identifier.doi | 10.3390/antibiotics8030103 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Antibiotics | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51941_1.pdf | 10,99 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons