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https://hdl.handle.net/1822/58787
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, C. F. | por |
dc.contributor.author | Babo, Pedro Miguel Sousa | por |
dc.contributor.author | Gulino, M. | por |
dc.contributor.author | Costa, Lígia Francisca Rodrigues | por |
dc.contributor.author | Oliveira, J. M. | por |
dc.contributor.author | Silva-Correia, Joana | por |
dc.contributor.author | Domingues, Rui Miguel Andrade | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.date.accessioned | 2019-01-30T15:36:59Z | - |
dc.date.available | 2019-01-30T15:36:59Z | - |
dc.date.issued | 2018-09 | - |
dc.date.submitted | 2018-03 | - |
dc.identifier.citation | Silva C. F., Babo P. S., Gulino M., Costa L., Oliveira J. M., Silva-Correia J., Domingues R. M. A., Reis R. L., Gomes M. E. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration, Acta Biomaterialia, doi:10.1016/j.actbio.2018.07.035, 2018 | por |
dc.identifier.issn | 1742-7061 | por |
dc.identifier.uri | https://hdl.handle.net/1822/58787 | - |
dc.description.abstract | Bioengineered soft tissues on any meaningful scale or complexity must incorporate aspects of the functional tissue, namely a vasculature, providing cells oxygen and nutrients critical for their survival. However, the ability of tissue engineering strategies to promote a fast revascularization is critically limited. Particularly in endodontic regenerative therapies, the complicated anatomy of the root canal system, and the narrow apical access limit the supply of new blood vessels and pulp tissue ingrowth. Here we characterize the viscoelastic and microstructural properties of a class of injectable hyaluronic acid (HA) hydrogels formed in situ, reinforced with cellulose nanocrystals (CNCs) and enriched with platelet lysate (PL), and test its ability to promote cells recruitment and proangiogenic activity in vitro. The incorporation of CNCs enhanced the stability of the materials against hydrolytic and enzymatic degradation. Moreover, the release of the chemotactic and pro-angiogenic growth factors (GFs) (PDGF and VEGF) from the PL-laden hydrogels showed an improved sustained profile proportional to the amount of incorporated CNCs. The PL-laden hydrogels exhibited preferential supportive properties of encapsulated human dental pulp cells (hDPCs) in in vitro culture conditions. Finally, PL-laden hydrogels stimulated chemotactic and pro-angiogenic activity by promoting hDPCs recruitment and cell sprouting in hDPCs/human umbilical vein endothelial cell co-cultures in vitro, and in an ex vivo model. These results support the use of the combined system as a scaffold for GFs delivery and cells recruitment, thereby exhibiting great clinical potential in treating injuries in vascularized tissues. | por |
dc.description.sponsorship | RECOGNIZE project (UTAP-ICDT/CTM-BIO/0023/2014), project NORTE-01-0145-FEDER-000021. R.M.A. Domingues acknowledges FCT for SFRH/BPD/112459/2015. P.S. Babo acknowledges the project FOOD4CELLS (PTDC/CTM-BIO/4706/2014-POCI-01-0145-FEDER 016716) for his post-doc grant | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Dental pulp cells | por |
dc.subject | Endodontic tissue regeneration | por |
dc.subject | Growth factors-controlled release | por |
dc.subject | Injectable hydrogel | por |
dc.subject | Migration | por |
dc.subject | Neo-angiogenesis | por |
dc.title | Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S174270611830429X | por |
dc.comments | http://3bs.uminho.pt/node/19738 | por |
oaire.citationStartPage | 155 | por |
oaire.citationEndPage | 171 | por |
oaire.citationVolume | 77 | por |
dc.date.updated | 2019-01-30T09:50:20Z | - |
dc.identifier.eissn | 1878-7568 | por |
dc.identifier.doi | 10.1016/j.actbio.2018.07.035 | por |
dc.identifier.pmid | 30031163 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Acta Biomaterialia | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19738-Silva2018.pdf | 4,29 MB | Adobe PDF | Ver/Abrir |