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https://hdl.handle.net/1822/5864
Título: | Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
Autor(es): | Juraščík, Martin Guimarães, Pedro M. R. Klein, Jaroslav Domingues, Lucília Teixeira, J. A. Markoš, J. |
Palavras-chave: | Ethanol fermentation of lactose Recombinant saccharomyces cerevisiae Kinetic model Kinetic parameters estimation |
Data: | Ago-2006 |
Editora: | Wiley |
Revista: | Biotechnology and Bioengineering |
Citação: | "Biotechnology and bioengineering". ISSN 0006-3592. 94:6 (Aug. 2006) 1147-1154. |
Resumo(s): | This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol), and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass, and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab-scale fermentor with the initial lactose concentrations ranging from 5 g/dm³ to 50 g/dm³. A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass, and dissolved oxygen concentrations) was achieved. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/5864 |
DOI: | 10.1002/bit.20941 |
ISSN: | 0006-3592 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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BB2006_Jurascik[2].pdf | 293,83 kB | Adobe PDF | Ver/Abrir |