Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/56894
Título: | Flow cytometry and fluorescence microscopy as tools for structural and functional analysis of vacuoles isolated from yeast and plant cells |
Autor(es): | Rodrigues, Jorge M. P. Pereira, Cátia S. Fontes, Natacha Gerós, Hernâni Côrte-Real, Manuela |
Palavras-chave: | Isolated vacuoles Flow cytometry Fluorescence microscopy Yeast Plant cell |
Data: | 19-Jun-2018 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Citação: | Rodrigues, Jorge M. P.; Cátia S. Pereira; Fontes, Natacha; Gerós, Hernâni; Côrte-Real, Manuela, Flow cytometry and fluorescence microscopy as tools for structural and functional analysis of vacuoles isolated from yeast and plant cells. Plant Vacuolar Trafficking: Methods and Protocols, Vol. Methods in Molecular Biology 1789, Springer New York, 2018. ISBN: 978-1-4939-7856-4, 101-115 |
Resumo(s): | A series of optimized protocols to isolate vacuoles from both yeast and plant cells, and to characterize the purified organelles at a functional and structural level, are described. For this purpose, we took advantage of the combined use of cell fractionation techniques with different fluorescence-based approaches namely flow cytometry, fluorescence microscopy and spectrofluorimetry. These protocols altogether constitute valuable tools for the study of vacuole structure and function, as well as for the high-throughput screening of drug libraries to identify new molecules that target the vacuole. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/56894 |
ISBN: | 978-1-4939-7855-7 |
e-ISBN: | 978-1-4939-7856-4 |
DOI: | 10.1007/978-1-4939-7856-4_8 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/book/10.1007/978-1-4939-7856-4 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_47799_1.pdf Acesso restrito! | 538,96 kB | Adobe PDF | Ver/Abrir |