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dc.contributor.authorCosta, Ana Ritapor
dc.contributor.authorMilho, Catarinapor
dc.contributor.authorAzeredo, Joanapor
dc.contributor.authorPires, Diana Priscila Pensopor
dc.date.accessioned2018-10-22T20:41:34Z-
dc.date.issued2018-
dc.identifier.citationCosta, Ana Rita; Milho, C.; Azeredo, Joana; Pires, Diana P., Synthetic biology to engineer bacteriophage genomes. In Joana Azeredo, Sanna Sillankorva, Bacteriophage Therapy, Vol. Methods in Molecular Biology 1693, New York: Humana Press, 2018. ISBN: 978-1-4939-7394-1, 285-300por
dc.identifier.isbn978-1-4939-7394-1por
dc.identifier.issn1064-3745por
dc.identifier.urihttps://hdl.handle.net/1822/56504-
dc.description.abstractRecent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, targeted drug delivery, or even assembly of new materials. In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes are addressed: a yeast-based platform and bacteriophage recombineering of electroporated DNA.por
dc.description.sponsorshipThis work was supported by the Portuguese Foundation for Scienceand Technology (FCT)under the scope ofthe project PTDC/ BBB-BSS/6471/2014, the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145FEDER-006684), and under the scope of the Project RECI/ BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). This work was also supported by BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Ana Rita Costa, Catarina Milho and Diana Priscila Pires acknowledge FCT for the grants SFRH/BPD/94648/2013, SFRH/BD/94434/2013 and SFRH/BPD/116187/2016, respectively.por
dc.language.isoengpor
dc.publisherHumana Presspor
dc.relationPTDC/BBB-BSS/6471/2014por
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147337/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/COMPETE/126270/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F94648%2F2013/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F94434%2F2013/PTpor
dc.relationSFRH/BPD/116187/2016por
dc.rightsclosedAccesspor
dc.subjectBacteriophagepor
dc.subjectBacteriophage engineeringpor
dc.subjectYACpor
dc.subjectBREDpor
dc.titleSynthetic biology to engineer bacteriophage genomespor
dc.typebookPartpor
dc.peerreviewedyes-
dc.commentsCEB47177por
oaire.citationStartPage285por
oaire.citationEndPage300por
oaire.citationConferencePlaceNew York-
oaire.citationVolumeMethods in Molecular Biology 1693-
dc.date.updated2018-10-21T11:42:41Z-
dc.identifier.doi10.1007/978-1-4939-7395-8_21por
dc.identifier.pmid29119447por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
sdum.journalMethods in Molecular Biologypor
sdum.bookTitleBacteriophage Therapypor
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