Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/56417
Título: | Power analysis of single-cell RNA-sequencing experiments |
Autor(es): | Svensson, Valentine Natarajan, Kedar Nath Ly, Lam-Ha Miragaia, Ricardo J. Labalette, Charlotte Macaulay, Iain C. Cvejic, Ana Teichmann, Sarah A. |
Palavras-chave: | Gene expression RNA sequencing Transcriptomics |
Data: | 6-Mar-2017 |
Editora: | Springer Nature |
Revista: | Nature Methods |
Citação: | Svensson, Valentine; Natarajan, Kedar Nath; Ly, Lam-Ha; Miragaia, Ricardo J.; Labalette, Charlotte; Macaulay, Iain C.; Cvejic, Ana; Teichmann, Sarah A., Power analysis of single-cell RNA-sequencing experiments. Nature Methods, 14(4), 381-387, 2017 |
Resumo(s): | Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and providing insights into developmental processes and transcriptional stochasticity. A key question is how the variety of available protocols compare in terms of their ability to detect and accurately quantify gene expression. Here, we assessed the protocol sensitivity and accuracy of many published data sets, on the basis of spike-in standards and uniform data processing. For our workflow, we developed a flexible tool for counting the number of unique molecular identifiers (https://github.com/vals/umis/). We compared 15 protocols computationally and 4 protocols experimentally for batch-matched cell populations, in addition to investigating the effects of spike-in molecular degradation. Our analysis provides an integrated framework for comparing scRNA-seq protocols. |
Tipo: | Artigo |
Descrição: | All data generated in this study have been deposited in the ArrayExpress database under accession codes E-MTAB-5480, E-MTAB-5481, E-MTAB-5482, E-MTAB-5483, E-MTAB-5484, E-MTAB-5485, and E-MTAB-5486. Summary tables are provided as supplementary files. |
URI: | https://hdl.handle.net/1822/56417 |
DOI: | 10.1038/nmeth.4220 |
ISSN: | 1548-7091 |
e-ISSN: | 1548-7105 |
Versão da editora: | http://www.nature.com/nmeth/index.html |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_46719_1.pdf Acesso restrito! | 1,4 MB | Adobe PDF | Ver/Abrir |