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https://hdl.handle.net/1822/56374
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Campo DC | Valor | Idioma |
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dc.contributor.author | Sousa, C. A. | por |
dc.contributor.author | Soares, Helena M. V. M. | por |
dc.contributor.author | Soares, Eduardo V. | por |
dc.date.accessioned | 2018-10-18T16:52:19Z | - |
dc.date.issued | 2018-07-25 | - |
dc.identifier.citation | Sousa, C. A.; Soares, Helena MVM; Soares, Eduardo V., Nickel oxide (NiO) nanoparticles induce loss of cell viability in yeast mediated by oxidative stress. Chemical Research in Toxicology, 31(8), 658-665, 2018 | por |
dc.identifier.issn | 0893-228X | por |
dc.identifier.uri | https://hdl.handle.net/1822/56374 | - |
dc.description.abstract | The present work aimed to elucidate whether the toxic effects of nickel oxide (NiO) nanoparticles (NPs) on the yeast Saccharomyces cerevisiae are associated with oxidative stress (OS) and what mechanisms may contribute to this OS. Cells exposed to NiO NPs accumulated superoxide anions and hydrogen peroxide, which are intracellularly generated. Yeast cells co-exposed to NiO NPs and antioxidants (L-ascorbic acid and N-tert-butyl--phenylnitrone) showed quenching of reactive oxygen species (ROS) and increased resistance to NiO NPs, indicating that the loss of cell viability was associated with ROS accumulation. Mutants lacking mitochondrial DNA (0) displayed reduced levels of ROS and increased resistance to NiO NPs, suggesting the involvement of the mitochondrial respiratory chain in ROS production. Yeast cells exposed to NiO NPs presented decreased levels of reduced glutathione (GSH). Mutants deficient in GSH1 (gsh1) or GSH2 (gsh2) genes displayed increased levels of ROS and increased sensitivity to NiO NPs, which underline the central role of GSH against NiO NPs-induced OS. This work suggests that the increased levels of intracellular ROS (probably due to the perturbation of the electron transfer chain in mitochondria) combined with the depletion of GSH pool constitute important mechanisms of NiO NPs-induced loss of cell viability in the yeast S. cerevisiae. | por |
dc.description.sponsorship | This work was performed in the framework of the financing by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01−0145FEDER-006684), BioTecNorte operation (NORTE-01−0145FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and LAQV (UID/QUI/50006/2013 - POCI/01/0145/FEDER/007265) with financial support from FCT/MEC through national funds and cofinanced by FEDER, under the Partnership Agreement PT2020. C.A.S. gratefully acknowledges the doctoral grant (SFRH/BD/101452/2014) from FCT. | por |
dc.language.iso | eng | por |
dc.publisher | American Chemical Society | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147218/PT | por |
dc.relation | SFRH/BD/101452/2014 | por |
dc.rights | restrictedAccess | por |
dc.title | Nickel oxide (NiO) nanoparticles induce loss of cell viability in yeast mediated by oxidative stress | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://pubs.acs.org/journal/crtoec | por |
dc.comments | CEB47889 | por |
oaire.citationStartPage | 658 | por |
oaire.citationEndPage | 665 | por |
oaire.citationIssue | 8 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationVolume | 31 | por |
dc.date.updated | 2018-10-18T10:04:31Z | - |
dc.identifier.eissn | 1520-5010 | por |
dc.identifier.doi | 10.1021/acs.chemrestox.8b00022 | por |
dc.identifier.pmid | 30043610 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Chemical Research in Toxicology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_47889_1.pdf Acesso restrito! | 2,02 MB | Adobe PDF | Ver/Abrir |