Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/56333
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Gonçalves, Filipa Daniela Gomes | por |
dc.contributor.author | Castro, T. | por |
dc.contributor.author | Azóia, Nuno G. | por |
dc.contributor.author | Ribeiro, Artur | por |
dc.contributor.author | Silva, Carla Manuela Pereira Marinho da | por |
dc.contributor.author | Cavaco-Paulo, Artur | por |
dc.date.accessioned | 2018-10-18T10:40:32Z | - |
dc.date.available | 2018-10-18T10:40:32Z | - |
dc.date.issued | 2018-10-04 | - |
dc.date.submitted | 2018-01 | - |
dc.identifier.citation | Gonçalves, Filipa D.; Castro, T.; Azoia, Nuno G.; Ribeiro, Artur; Silva, Carla; Cavaco-Paulo, Artur, Two Engineered OBPs with opposite temperature-dependent affinities towards 1-aminoanthracene. Scientific Reports, 8(14844), 2018 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/56333 | - |
dc.description.abstract | Engineered odorant-binding proteins (OBPs) display tunable binding affinities triggered by temperature alterations. We designed and produced two engineered proteins based on OBP-I sequence: truncated OBP (tOBP) and OBP::GQ20::SP-DS3. The binding affinity of 1-aminoanthracene (1-AMA) to these proteins revealed that tOBP presents higher affinity at 25°C (kd=0.45M) than at 37°C (kd=1.72M). OBP::GQ20::SP-DS3 showed an opposite behavior, revealing higher affinity at 37°C (kd=0.58M) than at 25°C (kd=1.17M). We set-up a system containing both proteins to evaluate their temperature-dependent binding. Our data proved the 1-AMA differential and reversible affinity towards OBPs, triggered by temperature changes. The variations of the binding pocket size with temperature, confirmed by molecular modelling studies, were determinant for the differential binding of the engineered OBPs. Herein we described for the first time a competitive temperature-dependent mechanism for this class of proteins. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684). A.R. and F.G. thank FCT for funding their scholarships with the references SFRH/BPD/98388/2013 and SFRH/BD/114684/2016, respectively. T.G.C. thanks senior position funded by the European Union through the European Regional Development Fund (ERDF) under the Competitiveness Operational Program (COP-A1-A1.1.4-E nr.30/01.09.2016). C.S. thanks to the BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Access to computing resources funded by the Project "Search-ON2: Revitalization of HPC infrastructure of UMinho" (NORTE-07-0162-FEDER-000086), co-funded by the North Portugal Regional Operational Programme (ON.2 - O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF), is also gratefully acknowledged. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Group | por |
dc.rights | openAccess | por |
dc.title | Two Engineered OBPs with opposite temperature-dependent affinities towards 1-aminoanthracene | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-018-33085-8 | por |
dc.comments | CEB48973 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 8 | por |
dc.date.updated | 2018-10-18T10:04:00Z | - |
dc.identifier.eissn | 2045-2322 | por |
dc.identifier.doi | 10.1038/s41598-018-33085-8 | por |
dc.identifier.pmid | 30287882 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_48973_1.pdf | 3,99 MB | Adobe PDF | Ver/Abrir |