Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/56295
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Novoa-Carballal, R. | por |
dc.contributor.author | Carretero, A. C. | por |
dc.contributor.author | Pacheco, R. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Pashkuleva, I. | por |
dc.date.accessioned | 2018-10-17T17:27:36Z | - |
dc.date.available | 2019-08-01T06:00:10Z | - |
dc.date.issued | 2018-07 | - |
dc.date.submitted | 2018-07 | - |
dc.identifier.citation | Novoa-Carballal R., Carretero A. C., Pacheco R., Reis R. L., Pashkuleva I. Star-like glycosaminoglycans with superior bioactivity assemble with proteins into microfibers, Chemistry: A European Journal , Vol. 24, pp. 14341 - 14345, doi:10.1002/chem.201802243, 2018 | por |
dc.identifier.issn | 0947-6539 | por |
dc.identifier.uri | https://hdl.handle.net/1822/56295 | - |
dc.description.abstract | We show that glycosaminoglycans (GAGs) with high molecular weight can be grafted via their reducing end on hyper-branched synthetic cores by oxime condensation without the need of any previous functionalisation of the polysaccharide. The versatility of this reaction is demonstrated by the use of hyaluronan, chondroitin sulfate and heparin with up to 60 sugar units. The isothermal calorimetry analysis demonstrated that the generated star-like glycopolymers have superior bioactivity. Moreover, when mixed with positively charged proteins (e.g. FGF-2) they form microfiber structures instead of the spherical nanocomplexes described for linear GAGs. Our results suggest that the described star-like GAG are closer mimics of the proteoglycans at structural and functional level and therefore have a huge potential in the development of tissue engineering platforms and therapeutics by modulating the activity and presentation of various proteins such as growth factors. | por |
dc.description.sponsorship | This work was supported by EC (POLARIS REGPOT-20122013-1-316331; ComplexiTE ERC-2012-ADG 20120216321266; and H2020-TWIN-CHEM2NATURE-692333 POLARIS). The authors thank to Marcelo Calderon and Ana Sousa Herves from the Freie Universität Berlin for providing the hPG and to Diana Soares da Costa for the images acquired by confocal microscopy. RNC and IP are thankful to the Portuguese Foundation for Science and Technology (IF00032/2013 and IF/00373/2014). ACC acknowledges CAPES for grant BEX: 12926-13/2. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-VCH | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/316331/EU | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/321266/EU | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/692333/EU | por |
dc.rights | openAccess | por |
dc.subject | glycopolymers | por |
dc.subject | Glycosaminoglycans | por |
dc.subject | hyperbranched polymers | por |
dc.subject | isothermal calorimetry | por |
dc.subject | self-assembly | por |
dc.subject | fibroblast growth factor-2 | por |
dc.subject | heparin | por |
dc.subject | oxime | por |
dc.title | Star-like glycosaminoglycans with superior bioactivity assemble with proteins into microfibers | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/epdf/10.1002/chem.201802243 | por |
dc.comments | http://3bs.uminho.pt/node/19561 | por |
oaire.citationStartPage | 14341 | por |
oaire.citationEndPage | 14345 | por |
oaire.citationIssue | 54 | por |
oaire.citationVolume | 24 | por |
dc.date.updated | 2018-10-17T15:08:21Z | - |
dc.identifier.doi | 10.1002/chem.201802243 | por |
dc.identifier.pmid | 29974983 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Chemistry - A European Journal | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19561-Novoa-Carballal_et_al-2018-Chemistry_-_A_European_Journal.pdf | 1,9 MB | Adobe PDF | Ver/Abrir |