Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/56280
Título: | Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation |
Autor(es): | Gonçalves, A. I. Rotherham, M. Markides, H. Rodrigues, M. T. Reis, R. L. Gomes, M. E. Haj, A. J. |
Palavras-chave: | Activin A receptor Human adipose derived stem cells Magnetic nanoparticles Tendon Tissue Engineering TGF-β/Smad2/3 signaling pathway Activin A receptor TGF-beta/Smad2/3 signaling pathway |
Data: | Jun-2018 |
Editora: | Elsevier 1 |
Revista: | Nanomedicine: Nanotechnology, Biology and Medicine |
Citação: | Gonçalves A. I., Rotherham M., Markides H., Rodrigues M. T., Reis R. L., Gomes M. E., Haj A. E. Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation, Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 14, Issue 4, pp. 1149-1159, doi:10.1016/j.nano.2018.02.008, 2018 |
Resumo(s): | Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor. Upon activation, the phosphorylation of Smad2/3 is induced allowing translocation of the complex to the nucleus, regulating tenogenic transcriptional responses. Our study demonstrates the potential remote activation of MNPs tagged hASCs to trigger the Activin receptor leading to tenogenic differentiation. These results may provide insights toward tendon regeneration therapies. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/56280 |
DOI: | 10.1016/j.nano.2018.02.008 |
ISSN: | 1549-9634 |
Versão da editora: | http://www.nanomedjournal.com/article/S1549-9634(18)30041-8/fulltext |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19387-GonçalvesAI_Nanomed 2018.pdf | 3,02 MB | Adobe PDF | Ver/Abrir |