Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/55739
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Amorim, Cláudia | por |
dc.contributor.author | Silvério, Sara Isabel Cruz | por |
dc.contributor.author | Silva, Soraia P. | por |
dc.contributor.author | Coelho, Elisabete | por |
dc.contributor.author | Coimbra, Manuel A. | por |
dc.contributor.author | Prather, Kristala L. J. | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2018-09-05T09:48:01Z | - |
dc.date.available | 2018-09-05T09:48:01Z | - |
dc.date.issued | 2018-11 | - |
dc.identifier.citation | Amorim, Cláudia; Silvério, Sara C.; Silva, Soraia P.; Coelho, Elisabete; Coimbra, Manuel A.; Prather, Kristala L. J.; Rodrigues, Lígia R., Single-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers spent grain. Carbohydrate Polymers, 199, 546-554, 2018 | por |
dc.identifier.issn | 0144-8617 | por |
dc.identifier.uri | https://hdl.handle.net/1822/55739 | - |
dc.description.abstract | Brewers spent grain (BSG) is an inexpensive and abundant brewery by-product that can be used to produce prebiotic arabino-xylooligosaccharides (AXOS). In this study, Bacillus subtilis 3610 was used, for the first time, to produce AXOS through direct fermentation of BSG. Additionally, the microorganism was genetically modified to improve the AXOS production. The xylanase gene xyn2 from Trichoderma reesei coupled with a secretion tag endogenous to B. subtilis was cloned in pDR111 and integrated into its chromosome. After optimization by experimental design, AXOS with a degree of polymerization ranging from 2 to 6 were obtained. The maximum production yield expressed in xylose equivalents per amount of BSG (54.2 ± 1.1 mg/g) represents an increase of 33% comparing to the wild type. When compared with the enzymatic hydrolysis process, single-step fermentation with B. subtilis proved to be a very promising low-cost strategy for the simultaneous production of AXOS and valorization of BSG. | por |
dc.description.sponsorship | CA, SCS, EC and LRR acknowledge their grants (PD/BD/105870/ 2014, SFRH/BPD/88584/2012, SFRH/BPD/70589/2010 and SFRH/ BSAB/142873/2018) from Portuguese Foundation for Science and Technology(FCT).Thestudyreceived financialsupportfromFCTunder the scope of the strategic funding of UID/BIO/04469/2013 unit; COMPETE2020 (POCI-01-0145-FEDER-006684); QOPNAresearch Unit (FCT UID/QUI/00062/2013), through national founds and where applicable co-financed by the FEDER, within the PT2020 Partnership Agreement; the projects MultiBiorefinery (POCI-01-0145-FEDER016403); FoSynBio (POCI-01-0145-FEDER-029549) and Lignozymes (POCI-01-0145-FEDER-029773). The authors also acknowledge financial support from the BioTecNorte operation (NORTE-01-0145FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F88584%2F2012/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F70589%2F2010/PT | por |
dc.relation | SFRH/BSAB/142873/2018 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147415/PT | por |
dc.rights | openAccess | por |
dc.subject | Brewers spent grain | por |
dc.subject | prebiotic | por |
dc.subject | arabino-xylooligosaccharides | por |
dc.subject | xylanase | por |
dc.subject | Bacillus subtilis | por |
dc.subject | Trichoderma reesei | por |
dc.title | Single-step production of arabino-xylooligosaccharides by recombinant Bacillus subtilis 3610 cultivated in brewers spent grain | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/carbohydrate-polymers/ | por |
dc.comments | CEB47819 | por |
oaire.citationStartPage | 546 | por |
oaire.citationEndPage | 554 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 199 | por |
dc.date.updated | 2018-09-04T16:56:28Z | - |
dc.identifier.doi | 10.1016/j.carbpol.2018.07.017 | por |
dc.identifier.pmid | 30143161 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Carbohydrate Polymers | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47819_1.pdf | 680,73 kB | Adobe PDF | Ver/Abrir |