Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/55598
Título: | Wax-printed paper-based device for direct electrochemical detection of 3-nitrotyrosine |
Autor(es): | Martins, Gabriela Marques, Ana C. Fortunato, Elvira Sales, M. G. F. |
Palavras-chave: | Paper substrate 3-Nitrotyrosine Oxidative stress biomarker Electrochemical detection Point-of-care sensing |
Data: | 10-Set-2018 |
Editora: | Elsevier 1 |
Revista: | Electrochimica Acta |
Citação: | Martins, Gabriela; Marques, Ana C.; Fortunato, Elvira; Sales, M. G. F., Wax-printed paper-based device for direct electrochemical detection of 3-nitrotyrosine. Electrochimica Acta, 284, 60-68, 2018 |
Resumo(s): | An overview of the last trends in lab-on-chip technology highlights the continuous development of biosensing approaches in terms of simplicity, low-cost, and quick response, with a higher sensitivity and selectivity. In this context, electrochemical sensors arrived to overcome the need to develop small, accurate, portable and compact sensing devices, compatible with routine use in loco analysis, especially in point-of-care (POC) assessment. Herein, we have presented the first paper-based electrochemical sensor towards the detection of 3-nitrotyrosine (3-NT), a relevant oxidative stress biomarker. This innovative sensor was designed by, first, modifying paper to become a hydrophobic support and, second applying carbon and silver conductive inks to generate a three electrode-system on a small spot. An intensive voltammetric investigation of the paper-based screen-printed electrodes (SPEs), including the study of different redox-probes, scan-rate values, probe concentrations and supporting electrolyte solutions, confirmed their suitable and reliable electrochemical performance. Square wave voltammetry (SWV) was employed to assess the sensitivity of the proposed sensor at various concentrations of 3-NT, ranging from 500nM to 1mM, enabling a low detection limit (LOD) of 49.2nM. The interference of co-existing species was also tested and good selectivity against 3-NT was accomplished. Overall, this flexible paper-based platform constitutes a promising sensor material towards a quick, sensitive, cost-effective, sustainable and reproducible detection of 3-NT biomarker. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/55598 |
DOI: | 10.1016/j.electacta.2018.07.150 |
ISSN: | 0013-4686 |
e-ISSN: | 0013-4686 |
Versão da editora: | https://www.journals.elsevier.com/electrochimica-acta |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_47873_1.pdf Acesso restrito! | 1,9 MB | Adobe PDF | Ver/Abrir |