Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/54027
Título: | Expression, purification and in vitro biological activity from human recombinant BMP-2 produced by a novel approach |
Autor(es): | Bessa, Paulo C. Pedro, A. Nobre, Alexandra Dungl, P. Banerjee, A. Dopler, D. Klosch, B. Redl, H. Casal, Margarida Reis, R. L. |
Data: | 2006 |
Resumo(s): | Bone tissue engineering has been an increasing field of research during the last years. The ideal approach for a regenerative application would consist in the use of cells from the patient, scaffolding materials and differentiation growth factors. Bone morphogenetic protein-2 (BMP-2) is one such growth factors with a strong ability to induce new bone and cartilage formation and has been used as a powerful osteoinductive component of several late-stage tissue engineering products for bone grafting. In this work, we aimed at obtaining high yields of human recombinant BMP-2 in a stable, pure and biologically active form by use of a new bacteria expression system that circumvents the disadvantages of conventional recombinant protein preparation methods and to perform a study of the stability conditions and functionality of these peptides in vitro in human mesenchymal stem cells and C2C12 murine cell line. |
Tipo: | Poster em conferência |
URI: | https://hdl.handle.net/1822/54027 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Comunicações/Communications in Congresses |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1179410456_Poster ESF-EMBO.pdf | 1,46 MB | Adobe PDF | Ver/Abrir |