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https://hdl.handle.net/1822/52067
Título: | Stem cell-containing hyaluronic acid-based spongy hydrogels for integrated diabetic wound healing |
Autor(es): | Silva, Lucília Pereira Santos, T. C. Rodrigues, Daniel Barreira Pirraco, Rogério P. Cerqueira, Mariana Teixeira Reis, R. L. Correlo, V. M. Marques, A. P. |
Palavras-chave: | Diabetic foot ulcerations Spongy-like hydrogels Stem cells |
Data: | Mar-2017 |
Editora: | Elsevier 1 |
Revista: | Journal of Investigative Dermatology |
Citação: | da Silva L. P., Santos T. C., Rodrigues D. B., Pirraco R. P., Cerqueira M. T., Reis R. L., Correlo V. M., Marques A. P. Stem Cell-Containing Hyaluronic Acid-Based Spongy Hydrogels for Integrated Diabetic Wound Healing, Journal Of Investigative Dermatology, Vol. 137, pp. 1541-1551, doi:10.1016/j.jid.2017.02.976, 2017 |
Resumo(s): | The detailed pathophysiology of diabetic foot ulcers is yet to be established and improved treatments are still required. We propose a strategy that directs inflammation, neovascularization, and neoinnervation of diabetic wounds. Aiming to potentiate a relevant secretome for nerve regeneration, stem cells were precultured in hyaluronic acid-based spongy hydrogels under neurogenic/standard media before transplantation into diabetic mice full-thickness wounds. Acellular spongy hydrogels and empty wounds were used as controls. Reepithelialization was attained 4 weeks after transplantation independently of the test groups, whereas a thicker and more differentiated epidermis was observed for the cellular spongy hydrogels. A switch from the inflammatory to the proliferative phase of wound healing was revealed for all the experimental groups 2 weeks after injury, but a significantly higher M2(CD163 þ )/M1(CD86 þ ) subtype ratio was observed in the neurogenic preconditioned group that also failed to promote neoinnervation. A higher number of intraepidermal nerve fibers were observed for the unconditioned group probably due to a more controlled transition from the inflammatory to the proliferative phase. Overall, stem cell-containing spongy hydrogels represent a promising approach to enhance diabetic wound healing by positively impacting re-epithelialization and by modulating the inflammatory response to promote a successful neoinnervation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/52067 |
DOI: | 10.1016/j.jid.2017.02.976 |
ISSN: | 0022-202X |
e-ISSN: | 1523-1747 |
Versão da editora: | https://doi.org/10.1016/j.jid.2017.02.976 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19481-14_2017_LPS.pdf | 5,47 MB | Adobe PDF | Ver/Abrir |