Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/5127
Título: | A study on the immune response elicited in mice challenged with Staphylococcus epidermidis planktonic - or biofilm - grown cells |
Autor(es): | Cerca, Filipe Teixeira, Luzia Cerca, Nuno Madureira, Pedro Ribeiro, Adília Azeredo, Joana Vilanova, Manuel |
Data: | 2005 |
Editora: | Universidade Católica Portuguesa. Escola Superior de Biotecnologia |
Citação: | MICROBIOTEC, Póvoa de Varzim, Portugal, 2005 – “Micro’05-Biotec’05 : livro de actas do Congresso MICROBIOTEC’2005”. Porto : Escola Superior de Biotecnologia da Universidade Católica Portuguesa, 2005. p. 338. |
Resumo(s): | In this study a biofilm-forming strain of Staphylococcus epidermidis was used in order to evaluate, in a murine model, the immune response to bacteria grown either as planktonic cells or biofilm. Groups of male BALB/c mice were infected intra-peritoneally (i.p.), with 2×10^8 S. epidermidis cells per mice, obtained either from a mature biofilm (grown for 48h) or from a planktonic cell culture. Mice were sacrificed 6h, 24h and 8 days after the i.p. infection. Flow cytometric and qualitative cytospin analysis of cells recovered from peritoneal exsudates, 24h after infection, indicates that the bacterial cells obtained from the biofilm were less inflammatory than planktonic. Splenomegaly was observed in mice 8 days after the bacterial challenge, more pronounced in mice challenged with planktonic cells. Flow cytometric analysis of spleen cells showed in both groups of bacteria-challenged mice an increase in the number of lymphocyte cells, more marked in the animals challenged with planktonic cells. The isotypic profile of the imunoglobulins present in the sera of each group of mice was analysed by ELISA. Extracellular polymeric substance (EPS) isolated from a S. epidermidis biofilm grown for 48h inhibited in vitro nitrite production by macrophages stimulated with LPS and interferon-gamma. Our results indicate that S. epidermidis biofilm-grown cells were less inflammatory than planktonic cells, and suggest that EPS could diminish the inflammatory response elicited in the host by this bacterium. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/5127 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
microbiotec2005-338[1].pdf | 83,07 kB | Adobe PDF | Ver/Abrir |