Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/51165
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Sousa, Diana Zita Machado | por |
dc.contributor.author | Visser, Michael | por |
dc.contributor.author | van Gelder, Antonie H. | por |
dc.contributor.author | Boeren, Sjef | por |
dc.contributor.author | Pieterse, Mervin M. | por |
dc.contributor.author | Pinkse, Martijn W. H. | por |
dc.contributor.author | Verhaert, Peter D. E. M. | por |
dc.contributor.author | Vogt, Carsten | por |
dc.contributor.author | Franke, Steffi | por |
dc.contributor.author | Kümmel,Steffen | por |
dc.contributor.author | Stams, Alfons Johannes Maria | por |
dc.date.accessioned | 2018-02-27T23:47:28Z | - |
dc.date.available | 2018-02-27T23:47:28Z | - |
dc.date.issued | 2018-01-18 | - |
dc.identifier.citation | Sousa, Diana Z.; Visser, Michael; van Gelder, Antonie H.; Boeren, Sjef; Pieterse, Mervin M.; Pinkse, Martijn W. H.; Verhaert, Peter D. E. M.; Vogt, Carsten; Franke, Steffi; Kümmel,Steffen; Stams, A. J. M., The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways. Nature Communications, 9(239), 2018 | por |
dc.identifier.issn | 2041-1723 | por |
dc.identifier.uri | https://hdl.handle.net/1822/51165 | - |
dc.description.abstract | Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment. | por |
dc.description.sponsorship | Research was funded by grants of the Division of Chemical Sciences (CW-TOP 700.55.343) and Earth and Life Sciences (ALW 819.02.014) of The Netherlands Organisation for Scientific Research (NWO), the European Research Council (ERC grant 323009), and the Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Culture and Science | por |
dc.language.iso | eng | por |
dc.publisher | Springer Nature | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/323009/EU | por |
dc.rights | openAccess | por |
dc.title | The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.nature.com/ncomms/ | por |
dc.comments | CEB47388 | por |
oaire.citationIssue | 239 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 9 | por |
dc.date.updated | 2018-01-20T13:30:02Z | - |
dc.identifier.eissn | 2041-1723 | por |
dc.identifier.doi | 10.1038/s41467-017-02518-9 | por |
dc.identifier.pmid | 29339722 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Nature Communications | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47388_1.pdf | 1,1 MB | Adobe PDF | Ver/Abrir |