Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/51164
Título: | Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells |
Autor(es): | Ohradanova-Repic, Anna Nogueira, E. Hartl, Ingrid Gomes, Andreia C Preto, Ana Steinhuber, Eva Mühlgrabner, Vanessa Repic, Marko Kuttke, Mario Zwirzitz, Alexander Prouza, Marek Suchanek, Miloslav Wozniak-Knopp, Gordana Horejsi, Vaclav Schabbauer, Gernot Cavaco-Paulo, Artur Stockinger, Hannes |
Palavras-chave: | Active targeting Liposome functionalization Immunoliposome Antibody engineering Recombinant Fab antibody fragment |
Data: | 2018 |
Editora: | Elsevier 1 |
Revista: | Nanomedicine: Nanotechnology, Biology, and Medicine |
Citação: | Ohradanova-Repic, Anna; Nogueira, E.; Hartl, Ingrid; Gomes, Andreia C.; Preto, Ana; Steinhuber, Eva; Mühlgrabner, Vanessa; Repic, Marko; Kuttke, Mario; Zwirzitz, Alexander; Prouza, Marek; Suchanek, Miloslav; Wozniak-Knopp, Gordana; Horejsi, Vaclav; Schabbauer, Gernot; Cavaco-Paulo, Artur; Stockinger, Hannes, Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells. Nanomedicine-Nanotechnology Biology and Medicine, 14(1), 123-130, 2018. doi: 10.1016/j.nano.2017.09.003 |
Resumo(s): | Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/51164 |
DOI: | 10.1016/j.nano.2017.09.003 |
ISSN: | 15499634 |
e-ISSN: | 1549-9642 |
Versão da editora: | http://www.nanomedjournal.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46971_1.pdf | 1,06 MB | Adobe PDF | Ver/Abrir |